标题：来那度胺对肝癌SMMC-7721细胞VEGF表达的影响

      作者：孙静，任熙玲，曹珮华，武永红    (西安医学院医学技术系，陕西 西安 710021)

      卷次： 2018年29卷7期

      【摘要】 目的 探讨来那度胺对人肝癌SMMC-7721细胞的增殖抑制作用及其对肝癌细胞血管内皮细胞生长因子(VEGF)表达的影响。方法 体外培养人肝癌SMMC-7721细胞株，分别设空白对照组和不同浓度来那度胺处理组(25 μmol/L、50 μmol/L、100 μmol/L)。应用MTS细胞实验研究各组细胞的增殖率；采用ELISA和Western blot检测各组细胞VEGF的分泌和表达。结果 细胞增殖实验显示，与空白对照组相比，不同浓度来那度胺处理组(25 μmol/L、50 μmol/L、100 μmol/L)的增殖率分别为(78.50±6.41)%、(56.00±5.70)%、(32.51±3.52)%，差异具有统计学意义(P<0.05)；ELISA结果显示，空白对照组和不同浓度来那度胺处理组(25 μmol/L、50 μmol/L、100 μmol/L) VEGF的细胞外分泌水平分别为(88.00±4.40) pg/mL、(78.60±5.15) pg/mL、(58.27±9.10) pg/mL、(41.80±10.00) pg/mL，差异具有统计学意义(P<0.05)；Western blot结果显示，随着药物浓度增加，VEGF蛋白表达水平明显下调，差异具有统计学意义(P<0.05)。结论 来那度胺可能通过抑制肿瘤细胞生长、下调VEGF表达抗血管生成而发挥双重抗肿瘤的作用。
      【关键词】 来那度胺；肝癌；血管内皮生长因子；细胞增殖
      【中图分类号】 R735.7 【文献标识码】 A 【文章编号】 1003—6350（2018）07—0889—03

Effects of lenalidomide on vascular endothelial growth factor expression in hepatocellular carcinomaSMMC-7721 cells.
SUN Jing, REN Xi-ling, CAO Pei-hua, WU Yong-hong. Department of Medical Technology, Xi’anMedical College, Xi'an 710021, Shaanxi, CHINA
【Abstract】 Objective To investigate the inhibitory effect of lenalidomide on the proliferation of hepatocellularcarcinoma SMMC-7721 cells and its effects on vascular endothelial growth factor (VEGF) expression. Methods Hepa-tocellular carcinoma SMMC-7721 cells were cultured in vitro and divided into blank control group and different concen-trations of lenalidomide treatment group (25 μmol/L, 50 μmol/L, 100 μmol/L). The cell proliferation rate was examinedby MTS assay, and the VEGF secretion and expression were determined by ELISA and Western blot. Results Comparedwith the blank control group, the cell proliferation rate in 25 μmol/L, 50 μmol/L, 100 μmol/L lenalidomide treatmentgroups were (78.50±6.41)%, (56.00±5.70)%, (32.51±3.52)%, respectively (P<0.05), indicating that lenalidomide inhibit-ed the proliferation of tumor cells. ELISA results showed that the VEGF expression in blank control group, 25 μmol/L,50 μmol/L, 100 μmol/L lenalidomide treatment groups were (88.00±4.40) pg/mL, (78.60±5.15) pg/mL, (58.27±9.10) pg/mL,(41.80±10.00) pg/mL, respectively (P<0.05). Western blot showed that protein expression of VEGF were down-regulat-ed in SMMC-7721 cells treated by lnalidomide in a dose-dependent manner (P<0.05). Conclusion Lenalidomide mayplay its anticancer role through inhibiting the proliferation of SMMC-7721 cells in vitro and down-regulating VEGF ex-pression for angiogenesis．
      【Key words】 Lenalidomide; Hepatocellular carcinoma; Vascular endothelial growth factor (VEGF); Cell proliferation·论 著·doi:10.3969/j.issn.1003-6350.2018.07.001基金项目：2014年陕西省卫生计生委卫生科研项目(编号：2014D20)；陕西省教育厅2015年西安医学院专项科研计划(编号：15JK1627)；西安医学院2016年配套基金项目(编号：2016PT33)