标题：miR-106b对人肝癌细胞HepG2细胞迁移和侵袭能力的影响

      作者：嘉红云，黄思聪，覃楠，周强    (广州医科大学附属第二医院检验科，广东 广州 510260)

      卷次： 2018年29卷5期

      【摘要】 目的 探讨miR-106b对人肝癌细胞 HepG2迁移和侵袭能力的影响。方法 分别用阴性对照(NC组)、miR-106b模拟物(miR-106b组)和miR-106b抑制物(miR-106b-in组)转染HepG2细胞，实时 PCR检测三组HepG2细胞miR-106b的表达，划痕实验、Transwell实验、3D培养实验检测三组HepG2细胞的迁移及侵袭能力的差异。结果 实时PCR显示miR-106b组细胞miR-106b相对表达量为(17.83±1.66)，miR-106b-in组为(0.32±0.07)，差异有统计学意义(P<0.05)；NC组细胞 24 h及 48 h划痕修复率分别为(47±3)%、(71±6)%，miR-106b组分别为(65±5)%、(99±1)%，miR-106b-in组分别为(38±3)%、(56±4)%，组间两两比较差异均有统计学意义(P<0.05)；Transwell侵袭实验显示，NC组的穿室细胞数为(58±10)个，miR-106b组为(116±19)个，miR-106b-in组为(38±6)个，组间两两比较差异均有统计学意义(P<0.05)；3D培养实验显示，20个 200倍视野下，miR-106b组无伪足的球形细胞数为 (3±1)个，明显少于 NC组的 (7±2)个及 miR-106b-in组的 (11±2)个，差异均有统计学意义 (P<0.05)。 结论 miR-106b能明显增强HepG2细胞的迁移和侵袭能力。
      【关键词】 HepG2细胞；miR-106b；迁移；侵袭
      【中图分类号】 R735.7 【文献标识码】 A 【文章编号】 1003—6350（2018）05—0593—03

Effects of miR-106b on migration and invasion of HepG2 cell.
JIA Hong-yun, HUANG Si-cong, QIN Nan, ZHOUQiang. Department of Clinical Laboratory, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou510260, Guangdong, CHINA
【Abstract】 Objective To investigate the influence of miR-106b on HepG2 cell's migration and invasion.Methods HepG2 cells were respectively transfected with negative control (NC group), miR-106b analogue (miR-106bgroup) and miR-106b inhibitor (miR-106b-in group). Real-time PCR was used to detect the expression of miR-106b inHepG2 cells. The differences of HepG2 cells' migration and invasion in the three groups were detected and compared byWound Healing assay, Transwell assay and 3D spheroid invasion assay. Results Real-time PCR showed that the cells'miR-106b relative expression of the miR-106b group was (17.83 ± 1.66), which was significantly higher than (0.32 ±0.07) of the miR-106b-in group (P<0.05). The cells' 24 h and 48 h scratch repair rates of the NC group were (47±3)%and (71±6)% respectively, which were significantly lower than corresponding (65±5)% and (99±1)% of the miR-106bgroup (P<0.05). The Transwell experiment indicated that the migrated cells count in the NC group, the miR-106b groupand the miR-106b-in group were respectively (58±10), (116±19) and (38±6), and the differences among the three groupswere statistically significant (P<0.05) as well. Under 200 magnified visual field, 3D culture experiment expressed that thenumbers of globular cells without pseudopodia in the miR-106b group, miR-106b-in group, NC group were (3±1), (11±2),and (7±2), respectively, and the differences were statistically significant (P<0.05). Conclusion MiR-106b can signifi-cantly enhance HepG2 cell's migration and invasion.
      【Key words】 HepG2 cell; miR-106b; Migration; Invasiondoi:10.3969/j.issn.1003-6350.2018.05.001基金项目：广东省广州市科技和信息化局项目(编号：2014J4100063)