标题：VEGF、PCNA、p53及Survivin与膀胱移行细胞癌病理分级、临床分期的关系研究

      作者：熊涛，唐顺利，李凯，孙刚，张楚龙，朱晓霜，周庆文    (广东医科大学附属中山医院/中山市陈星海医院泌尿外科，广东 中山 528415)

      卷次： 2018年29卷3期

      【摘要】 目的 探讨膀胱移行细胞癌(BTCC)组织中血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)、p53基因及Survivin的阳性表达率，分析其与癌组织的病理分级和临床分期的相关性。方法 选择2015年1月至2017年5月经广东医科大学附属中山医院泌尿外科手术切除并经病理证实的BTCC组织石蜡标本67例(BTCC组)和正常膀胱黏膜组织标本 17例(正常膀胱组)，采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结(SP)法检测其VEGF、PCNA、p53及Survivin表达，比较BTCC与正常膀胱组织、不同病理分级及不同临床分期BTCC组织中各检测指标的阳性表达差异。结果 VEGF、PCNA、p53及Survivin在正常膀胱组中的阳性表达率均为0，在BTCC组中的阳性表达率依次为67.16%、68.66%、53.73%及74.63%，差异均有统计学意义(P<0.05)；VEGF、PCNA、p53及Survivin在G1组中的阳性表达率依次为 30.00%、25.00%、30.00%及 55.00%，在G2组中的阳性表达率依次为 73.33%、80.00%、53.33%及76.67%，在G3组中的阳性表达率依次为100.00%、100.00%、82.35%及94.12%，不同病理分级组之间比较差异均有统计学意义(P<0.05)；VEGF、PCNA、p53及Survivin在Tis~T1组中的阳性表达率依次为55.10%、57.14%、40.82%及 67.35%，均低于 T2~T4组的 100.00%、100.00%、88.89%及 94.44%，差异有统计学意义(P<0.05)。结论 VEGF、PCNA、p53及Survivin在正常膀胱黏膜组织中无阳性表达，在BTCC组织中的阳性表达率与病理分级、临床分期有关。
      【关键词】 膀胱移行细胞癌；血管内皮生长因子；增殖细胞核抗原；p53基因；Survivin基因
      【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350（2018）03—0342—04

Relationship between VEGF, PCNA, p53, Survivin and pathological grading, clinical staging of bladdertransitional cell carcinoma.
XIONG Tao, TANG Shun-li, LI Kai, SUN Gang, ZHANG Chu-long, ZHU Xiao-shuang,ZHOU Qing-wen. Department of Urology, the Affiliated Zhongshan Hospital of Guangdong Medical University/ChenxinghaiHospital of Zhongshan, Zhongshan 528415, Guangdong, CHINA
【Abstract】 Objective To explore the positive expression rate of the vascular endothelial growth factor (VEGF),proliferating cell nuclear antigen (PCNA), p53 gene and Survivin gene in bladder transitional cell carcinoma (BTCC), andanalyzed their relationship with the pathological grading and clinical staging of cancerous tissue. Methods A total of 67BTCC paraffin tissue samples (BTCC group) and 17 normal bladder paraffin tissue samples (normal bladder group)were resected and pathologically confirmed in Department of Urology, the Affiliated Zhongshan Hospital of Guang-dong Medical University from January 2015 to May 2017. Immunohistochemical streptavidin-perosidase (SP) methodwas used to detect the expression of VEGF, PCNA, p53 and Survivin. The difference of positive expression of eachtested index between normal bladder group and BTCC group of different pathological grading and clinical stagingwere compared. Results The positive expression rates of VEGF, PCNA, p53 and Survivin were all 0 in normal blad-der group, versus 67.16%, 68.66%, 53.73%, 74.63% in BTCC group, respectively (P<0.05). The positive expressionrates of VEGF, PCNA, p53 and Survivin were 30.00%, 25.00%, 30.00%, 55.00% in group G1 respectively, 73.33%,80.00%, 53.33%, 73.33% in group G2 and 100.00%, 100.00%, 82.35%, 94.12% in group G3, with statistically signifi-cant difference between each two groups (P<0.05). The positive expression rates of VEGF, PCNA, p53, Survivinwere 55.10%, 57.14%, 40.82%, 67.35% in group Tis-T1 respectively, which were significantly lower than 100.00%,·论 著·doi:10.3969/j.issn.1003-6350.2018.03.013