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      标题:脾多肽对幽门螺旋杆菌脂多糖诱导RAW264.7巨噬细胞TNF-α、IL-1β、IL-6及IL-17的作用
      作者:董启超,梁晖,胡聪,林旭,林智文    (珠海市人民医院普外一科,广东 珠海 519000)
      卷次: 2018年29卷1期
      【摘要】 目的 研究脾多肽对幽门螺旋杆菌脂多糖(LPS)诱导RAW264.7巨噬细胞炎症模型肿瘤坏死因子(TNF-α)、白介素(IL)-1β、IL-6及 IL-17的作用。方法 选取小鼠RAW264.7巨噬细胞为研究载体,分为空白组、模型组、脾多肽高、中和低浓度组,共五组,每组设3个复孔。采用幽门螺旋杆菌LPS诱导建立炎症细胞模型,予脾多肽给药干预,以37℃及5% CO2的条件下培养3 h,取上清样品备测TNF-α;再培养3 h,取上清样品备测 IL-1β、IL-6与 IL-17。采用酶联免疫法检测TNF-α、IL-1β、IL-6与 IL-17。结果 幽门螺旋杆菌脂多糖可以诱导RAW264.7巨噬细胞炎症模型。药物干预 3 h后,各组TNF-α水平比较,差异有统计学意义(P<0.05),脾多肽高、中、低浓度组TNF-α水平均明显低于模型组,差异有统计学意义(P<0.05)。高浓度组TNF-α水平均明显低于中、低浓度组,差异有统计学意义(P<0.05)。药物干预6 h后,各组 IL-1β、IL-6和 IL-17水平比较,差异有统计学意义(P<0.05)。脾多肽高、中剂量组 IL-1β、IL-6和 IL-17水平均明显低于模型组,差异有统计学意义(P<0.05)。结论 脾多肽能够减少幽门螺旋杆菌LPS介导的RAW264.7炎症细胞模型分泌促炎因子TNF-α、IL-1β、IL-6及 IL-17,其不仅以浓度依赖降低TNF-α含量水平,还能在炎症早期抑制TNF-α,从而减轻炎症反应的发展。
      【关键词】 脾多肽;幽门螺旋杆菌;脂多糖;细胞炎症模型
      【中图分类号】 R57 【文献标识码】 A 【文章编号】 1003—6350(2018)01—0001—03
Role of spleen polypeptides in the expression of INF-α, INF-1β, IL-6, IL-17 in RAW264.7 macrophage cellmodel induced by lipopolysaccharide of Helicobacter pylori. DONG Qi-chao, LIANG Hui, HU Cong, LIN Xu, LINZhi-wen. the First Department of General Surgery, Zhuhai People's Hospital, Zhuhai 519000, Guandong, CHINA
      【Abstract】 Objective To investigate the role of spleen polypeptides in the expression of tumour necrosis factorα (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-17 (IL-17) in RAW264.7 macrophage cell mod-el induced by the lipopolysaccharide (LPS). Methods RAW264.7 cells were used as the study model and divided into5 groups, including the blank group, model group, high dose, medium dose and low dose of spleen polypeptides group,and each group had 3 repetition. Inflammatory cell model was induced by the LPS of Helicobacter pylori. Then with thesituation of 37℃ and 5% CO2, each dose groups were treated with the spleen polypeptides. After 3 h of cell culture, thesupernatant was collected and TNF-α was tested. Then 3 more hours later, IL-1β, IL-6 and IL-17 were tested by en-zyme-linked immunesorbent assay (ELISA). Results The RAW264.7 macrophage inflammatory cell model could besuccessfully induced by LPS of Helicobacter pylori. After 3 h of cell culture, there were differences between the fivegroups in the concentration of TNF-α (P<0.05). Moreover, the concentration of TNF-α in each dose group of spleenpolypeptides was obviously lower than that in the model group (P<0.05). And the concentration of TNF-α in high dosegroup was remarkably lower than that in the medium and low dose groups (P<0.05). After 6 h of drug treating, therewere also significant differences between the five groups in the concentrations of IL-1β, IL-6 and IL-17 (P<0.05). Andthe concentrations of IL-1β, IL-6 and IL-17 in the high and medium dose groups were significantly lower than those inthe model group (P<0.05). Conclusion Spleen polypeptides can reduce the sections of TNF-α, IL-1β, IL-6 and IL-17from the RAW264.7 inflammatory cell model induced by the LPS of Helicobacter pylori, which is not only dose-depen-dent on decreasing the concentrations of TNF-α, but it can also inhibit the section in the early stage of inflammation alle-viating inflammatory process.
      【Key words】 Spleen polypeptides; Helicobacter pylori; Lipopolysaccharide; Inflammatory cell model·论 著·doi:10.3969/j.issn.1003-6350.2018.01.001

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