标题：P物质和 IL-1β在固定正畸牙龈沟液中的表达

      作者：闫利勇，刘焕燕，高静，张丽    (泰安市中心医院口腔科，山东 泰安 271000)

      卷次： 2017年28卷20期

      【摘要】 目的 通过检测固定正畸患者不同矫治时期龈沟液(GCF)中P物质(SP)和白细胞介素-1β (IL-1β)含量的动态变化，探讨两者在正畸牙牙周组织改建过程中的影响和临床意义。方法 选择 2015年4月至2016年3月于泰安市中心医院需要拔除双侧第一前磨牙固定矫治患者30例，随机选择一侧尖牙作为观察组，施加250 g远中移动初始力，另一侧为对照组不受力。分别在加力当天以及加力后1 d、7 d、14 d、21 d和28 d收集患者尖牙近中及远中龈沟液，采用酶联免疫吸附实验(ELISA)测定SP和 IL-1β含量。结果 正畸加力后观察组GCF中SP和 IL-1β均开始升高[(3.8±1.2) pg/mL、(42.4±11.5) ng/mL]，第7天均达到高峰[(5.0±1.4) pg/mL、(53.8±13.7) ng/mL]，第28天基本回落至基线[(2.6±0.8) pg/mL和(35.6±11.9) ng/mL]。对照组GCF中SP和IL-1β基本维持在基线。加力后1 d、7 d和14 d观察组GCF中SP含量和加力后1 d、7 d IL-1β含量与对照组比较差异有统计学意义(P<0.05)。结论 正畸牙龈沟液中SP和IL-1β的表达随牙周组织受力变化而变化，参与牙齿移动和牙槽骨改建，其监测为正确判断正畸牙的牙周状况提供一定参考依据。
      【关键词】 正畸牙移动；P物质；白细胞介素-1β；龈沟液
      【中图分类号】 R783.5 【文献标识码】 A 【文章编号】 1003—6350（2017）20—3331—03

Expression of Substance P and interleukin-1 β in gingival crevicular fluid during orthodontic treatment.
YANLi-yong, LIU Huan-yan, GAO Jing, ZHANG Li. Department of Stomatology, Tai’an City Central Hospital, Tai'an 271000,Shandong, CHINA
【Abstract】 Objective To investigate the dynamic changes of substance P (SP) and interleukin-1β (IL-1β) lev-els in gingival crevicular fluid (GCF), and to explore their influence and clinical significance on the construction of para-dentium during orthodontic toot movement. Methods The canines of 30 patients who admitted to Tai'an City CentralHospital from April 2015 to March 2016 and involving the extraction of the maxillary first premolar tooth were divid-ed randomly into two groups. The distal forces of 250 g were applied to the experimental canines and the other sideswere not. The GCF was collected before activation and at 1 d, 7 d, 14 d, 21 d and 28 d respectively after the initiationof the experiment. The levels of SP and IL-1β in GCF were determined by enzyme-linked immunosorbent assay (ELI-SA) tests. Results The levels of SP and IL-1β in the experimental group began to increase at 1 d from (3.8±1.2) pg/mLand (42.4±11.5) ng/mL, respectively, and reached the peak of (5.0±1.4) pg/mL and (53.8±13.7) ng/mL at 7 d after the ex-periment, then returned to baseline of (2.6±0.8) pg/mL and (35.6±11.9) ng/mL at 28 d. Both of them in the control groupmaintained at baseline level during the experiment. The changes of the level of SP at 1 d, 7 d, 14 d and IL-1β at 1 d, 7 dwere found statistically significant between the experimental and control groups (P<0.05). Conclusion The levels ofSP and IL-1β in GCF is correlated with orthodontic force, which may be involved in tooth movement and alveolar boneremodeling in response to mechanical stress, and therefore they can be used as a diagnostic efficient method for monitor-ing periodontal health in clinical practice.
      【Key words】 Orthodontic tooth movement; Substance P (SP); Interleukin -1β (IL-1β); Gingival crevicular fluid(GCF)·论 著·doi:10.3969/j.issn.1003-6350.2017.20.021