标题：去水淫羊藿素对大鼠视网膜缺血/再灌注损伤的保护作用

      作者：吕昊琴 1，吴维霖 1，刘艳芳 3，刘锦荣 1，刘星芳 1，谢佳丽 1，黄志华 2，王辉 3?    (1.赣南医学院研究生学院，江西 赣州 341000；2.赣南医学院生理学教研室，江西 赣州 341000；3.赣南医学院第一附属医院眼科，江西 赣州 341000)

      卷次： 2017年28卷18期

      【摘要】 目的 研究去水淫羊藿素(ICT)对大鼠视网膜缺血/再灌注损伤是否具有保护作用，并初步探讨其作用机制。方法 将40只SD大鼠采取随机数表法分为5组(n=8)，通过夹闭一侧颈总动脉的方法构建大鼠视网膜缺血/再灌注(I/R)模型。分别于夹闭前，一次性经腹腔注射不同剂量(1.0 mg/kg、2.0 mg/kg、4.0 mg/kg) ICT；假手术组及模型组给予等体积生理盐水腹腔注射；假手术组只分离不夹闭，模型组与药物组建立视网膜 I/R模型。各组分别在夹闭前、缺血30 min时行视网膜电流图(ERG)、眼底照相检查以及再灌注1 h后行ERG、眼底荧光血管造影(FFA)检查。再灌注6 h后获取视网膜，通过免疫印迹法检测白介素10 (IL-10)蛋白表达水平。结果 与模型组比较，ICT治疗组能显著改善ERG的a波、b波振幅降低，使后极部视网膜缺血动脉明显扩张，并且能升高视网膜 I/R损伤后视网膜组织 IL-10蛋白表达水平[(1.02±0.44) vs (0.47±0.21)]，差异有统计学意义(P<0.05)。结论 ICT对视网膜缺血/再灌注损伤具有保护作用，其保护机制可能与上调抗炎细胞因子IL-10蛋白表达水平，抑制炎症反应有关。
      【关键词】 去水淫羊藿素；视网膜；缺血再灌注损伤；IL-10；电流图
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2017）18—2932—04

Protective effects of icaritin on retinal ischemia/reperfusion injury in rats.
LV Hao-qin 1, WU Wei-lin 1, LIUYan-fang 3, LIU Jin-rong 1, LIU Xing-fang 1, XIE Jia-li 1, HUANG Zhi-hua 2, WANG Hui 3. 1. Graduate Faculty of GannanMedical University, Ganzhou 341000, Jiangxi, CHINA; 2. Department of Physiology, Gannan Medical University, Ganzhou341000, Jiangxi, CHINA; 3. Department of Ophthalmology, the First Affiliated Hospital of Gannan Medical University,Ganzhou 341000, Jiangxi, CHINA
【Abstract】 Objective To explore the protective effects of icaritin (ICT) on retinal ischemia/reperfusion injuryin rats and its potential mechanism. Methods Forty Sprague-Dawley rats were assigned into the five groups accordingto random number tables, with 8 cases in each group. The model of retinal ischemia/reperfusion injury was induced bythe ligation of the common carotid artery unilaterally. Respectively, these rats were randomized to receive intraperitonealinjection of saline, at the dosage of 1.0 mg/kg, 2.0 mg/kg, 4.0 mg/kg of ICT before the induction of retinal I/R injury.The rats in the sham group were isolated without ligation of the common carotid artery, and the retinal I/R model was es-tablished in the model group and the drug group. Electroretinography (ERG) and fundus photography were used to as-sess functional changes in retina before and 30 minutes after ligation. One hour after reperfusion, ERG and fluoresceinfundus angiography (FFA) were performed. After 6 h of reperfusion, the rats were sacrificed and the eyeballs were ex-tracted for retina. The expression of interleukin 10 (IL-10), one of anti-inflammatory mediators was quantified by West-ern blot analysis. Results Compared with the model group, the ICT groups ameliorated notably the decrease of the am-plitudes of the a- and b-waves and significantly dilate retinal vessels. Besides, the level of IL-10 in the retinal tissue afterretinal I/R damage increased, especially in 2.0 mg/kg ICT group (P<0.05). Conclusion ICT has a protective effect inretinal ischemia/reperfusion injury, possibly by increasing the expression of IL-10 and suppressing inflammation.
      【Key words】 Icaritin (ICT); Retinal; Ischemia/reperfusion injury; Interleukin 10 (IL-10); Electroretinography·论 著·