标题：低氧对间充质干细胞迁移及成骨分化的影响

      作者：黄年盛 1 综述 彭建强 2审校    (1.广东医学院附属福田人民医院，广东 深圳 518033；2.中山大学附属第八医院，广东 深圳 518033)

      卷次： 2017年28卷14期

      【摘要】 间充质干细胞(MSCs)由于具有来源广泛、自我更新及多向分化能力，是骨组织工程上的重要种子细胞。MSCs在体内的生理环境及植入后都是一个低氧状态，低氧是影响MSCs迁移、成骨分化的一个重要因素。大部分研究认为低氧通过调控相关趋化因子及细胞因子等促进MSCs向低氧处迁移，涉及的相关因子包括整合素家族、基质金属蛋白酶、Rho-GTPase家族、SDF-1α/CXCR4信号、OPN/CD44信号等。低氧诱导因子-1α (HIF-1α)信号可促进骨形成、骨修复，然而，低氧对MSCs成骨分化的影响尚存在较大争议，低氧环境下BMP-Smads、WNT/β-catenin、Notch、Hedgehog等成骨相关通路的变化是影响MSCs成骨分化的因素之一。本文就低氧对MSCs迁移及成骨分化的影响及机制做一综述。
      【关键词】 低氧；低氧诱导因子-1α；间充质干细胞；迁移；成骨分化；成骨相关通路
      【中图分类号】 R329.2+7 【文献标识码】 A 【文章编号】 1003—6350（2017）14—2333—05

Effects of hypoxia on migration and osteogenic differentiation of mesenchymal stem cells.
HUANG Nian-sheng 1,PENG Jian-qiang 2, 1. Futian People's Hospital Affiliated to Guangdong Medical University, Shenzhen 518033,Guangdong, CHINA; 2. Department of Spinal Surgery, the Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen518033, Guangdong, CHINA【Abstract】 Due to the extensive sources, self-renewal and ability of multi-directional differentiation, mesenchy-mal stem cells (MSCs) are essential seed cells for bone tissue engineering. The environment after transplantation andphysiological environment in vivo where MSCs live are a hypoxic state. Hypoxia is an important factor affecting MSCsmigration and osteogenic differentiation. Most research suggests that hypoxia promotes the migration of MSCs by regu-lating the related chemotactic factors and cytokines. The relevant factors include integrin family, matrix metalloproteinas-es, Rho-GTPase family, SDF-1α/CXCR4 signal, OPN/CD44 signal. However, the effect of hypoxia on the osteogenicdifferentiation of MSCs remains controversial. The variations of signaling pathways of BMP-Smads, WNT/β-catenin,Notch, Hedgehog in hypoxia may be one of the factors that influence the osteogenic differentiation of MSCs. In this pa-per, the effects of hypoxia on the migration and osteogenic differentiation of MSCs and its mechanism will be discussed.
      【Key words】 Hypoxia; HIF-1α; Mesenchymal stem cells; Migration; Osteogenic differentiation; Signaling pathway·综 述·doi:10.3969/j.issn.1003-6350.2017.14.030