标题：精子DNA完整性对复发性流产的影响

      作者：崔蓉，钟兴明，苗竹林，李月华，朱胜辉，姜彦嘉，杨宁，韦相才    (广东省计划生育科学技术研究所，广东 广州 510600)

      卷次： 2017年28卷12期

      【摘要】 目的 探讨精子DNA完整性与复发性流产的相关性。方法 病例来自于2015年1月至2016年6月在我院流产门诊就诊的夫妇，选取复发性流产患者丈夫作为研究组(n=37)和正常生育男性作为对照组(n=30)。取两组精液进行常规分析，同时采用精子染色质结构分析法(SCSA)对研究对象精子DNA完整性进行检测。结果 复发性流产患者丈夫组与正常生育男性组精液的量[(3.61±0.8) mL vs (3.74±0.7) mL]、精子浓度[(130.86±69.47)×106/mL vs(141.97±80.44)×106/mL]、精子活力[(54.18±11.75) % vs (56.33±11.78) %]以及正常形态精子百分率[(13.19±8.93) % vs(14.27±8.78) %]比较差异均无统计学意义(P>0.05)；复发性流产患者丈夫组精子DNA碎片指数为(28.33±7.97)%，低于正常生育男性组的(12.73±5.07)%，差异有显著统计学意义(P<0.01)。结论 精子DNA完整性损伤可能是复发性流产的相关因素，SCSA对评估生育力具有临床意义。
      【关键词】 复发性流产；精子DNA；完整性；精子染色质结构
      【中图分类号】 R714.21 【文献标识码】 A 【文章编号】 1003—6350（2017）12—2034—03

Effects of sperm DNA integrity on recurrent spontaneous abortions.
CUI Rong, ZHONG Xing-ming, MIAO Zhu-lin,LI Yue-hua, ZHU Sheng-hui, JIANG Yan-jia, YANG Ning, WEI Xiang-cai. Institute of Family Planning Science andTechnology of Guangdong Province, Guangzhou 510600, Guangdong, CHINA
【Abstract】 Objective To investigate the correlation between sperm DNA integrity and recurrent spontaneousabortions (RSA). Methods A total of 67 pairs of couple, who admitted to our abortion clinic from January 2015 to June2016, were selected as the research subjects. The husbands of RSA patients (n=37) were enrolled as the research group,and the husbands of healthy fertility women (n=30) were taken as the control group. The sperm of the two groups wereanalyzed by conventional method, and at the same time sperm DNA integrity was identified by sperm chromatin struc-ture analysis (SCSA). Results The semen volume, sperm concentration, sperm vigor and normal sperm morphologypercent of the research group were (3.61±0.8) mL, (130.86±69.47)×106/mL, (54.18±11.75)%, (13.19±8.93)%, respective-ly, which showed no significant difference with (3.74 ± 0.7) mL, (141.97 ± 80.44) × 106/mL, (56.33 ± 11.78)% , (14.27 ±8.78)% of the control group (P>0.05). The DNA fractionation index (DFI) of the research group was (28.33±7.97)%,which was significantly lower than (12.73±5.07)% of the control group (P<0.01). Conclusion The damage of spermDNA integrity may be associated with RSA. SCSA has a certain clinical significance in assessing fertility.
      【Key words】 Recurrent spontaneous abortions (RSA); Sperm DNA; Integrity; Sperm chromatin structure·临床经验·doi:10.3969/j.issn.1003-6350.2017.12.048基金项目：广东省医学科学技术研究基金(编号：A2014202)