标题：胰岛素样生长因子-1在大鼠重症急性胰腺炎中的作用

      作者：王力波 1，杨志文 2，王静 2，翁成钊 2，徐萍 2    (1.南京医科大学，江苏 南京 210029；2.南京医科大学附属上海市松江区中心医院消化内科，上海 201600)

      卷次： 2017年28卷4期

      【摘要】 目的 观察胰岛素样生长因-1 (IGF-1)在重症急性胰腺炎(SAP)大鼠中的治疗作用。方法 将30只SD大鼠按随机数字表法分为正常对照组、假手术组、SAP组、高剂量 IGF-1组和低剂量 IGF-1组，每组6只。采用改良的Aho法制作SAP模型，在造模6 h后处死大鼠，收集胰腺组织、外周血和腹水。采用酶联免疫吸附试验(ELISA)检测各组大鼠血清细胞因子(IL-1β、IL-6)表达。Real-time PCR方法检测胰腺组织(TLR-4、MAPK p38、NF-κB p65)mRNA转录水平及蛋白水平变化。结果 SAP组大鼠腹水量、转氨酶、血清及腹水淀粉酶和炎症细胞因子 IL-1β、IL-6较正常对照组及假手术组显著升高，差异均有统计学意义(P<0.05)；经 IGF-1干预后，大鼠腹水量、转氨酶、血清及腹水淀粉酶、IL-1β和 IL-6较SAP组明显下降，且随 IGF-1剂量的增加，大鼠腹水量、转氨酶、血清及腹水淀粉酶、IL-1β和IL-6下降更加显著，差异均有统计学意义(P<0.05)。结论 IGF-1能有效减轻SAP大鼠胰腺炎的病情。
      【关键词】 胰岛素样生长因-1；重症急性胰腺炎；大鼠
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2017）04—0517—05

Effects of insulin-like growth factor-1 in the treatment of severe acute pancreatitis.
WANG Li-bo 1, YANG Zhi-wen 2,WANG Jing 2, WENG Cheng-zhao 2, XU Ping 2. 1. Nanjing Medical University, Nanjing 210029, Jiangsu, CHINA; 2.Department of Gastroenterology, Shanghai Songjiang District Central Hospital Affiliated to Nanjing Medical University,Shanghai 201600, CHINA
【Abstract】 Objective To observe the effects of insulin-like growth factor-1 (IGF-1) in the treatment of severeacute pancreatitis (SAP). Methods Thirty SD rats were randomly divided into five equal groups (n=6 each): normalgroup, sham operation group, SAP group, high dose IGF-1 group and low dose IGF-1 group. The SAP model was estab-lished by the modified Aho method. Rats were sacrificed 6 h after modeling for collection of pancreatic tissues, peripher-al blood and ascites. The expression status of the serum cytokines (IL-1β, IL-6) was detected by enzyme-linked immu-nosorbent assay (ELISA). Real-time PCR was used to detect the changes in TLR-4, MAPK p38 and NF-κB p65 mRNAand protein levels in pancreatic tissues. Results In the SAP group, there were significant increases in ascites volume,transaminases, serum and ascites amylase, IL-1β and IL-6, compared with those in the normal group and control group(P<0.05). IGF-1 intervention resulted in significant decreases in ascites volume, transaminases, serum and ascites amy-lase, IL-1βand IL-6, compared to those in the SAP group, and there were greater decreases in ascites volume, transami-nases, serum and ascites amylase, IL-1β and IL-6 with increasing IGF-1 dose (P<0.05). Conclusion IGF-1 could ef-fectively alleviate pancreatitis in SAP rats.
      【Key words】 Insulin-like growth factor-1; Severe acute pancreatitis; Ratsdoi:10.3969/j.issn.1003-6350.2017.04.001基金项目：南京医科大学科技发展基金资助基金(编号：2012-III-58）