标题：FOXO1在乳腺癌中的表达及临床意义

      作者：陈宝英 1，张英 1，黄胜超 2，颜泽铭 2，何敢 2    (广东医学院附属医院肿瘤中心 1、乳腺科 2，广东 湛江 524001)

      卷次： 2017年28卷9期

      【摘要】 目的 探讨 FOXO1在乳腺癌发生发展过程中的作用及其临床意义。方法 选取2012年6月至2013年1月在广东医学院附属医院经病理证实的乳腺癌组织、癌旁正常乳腺组织、乳腺良性病变组织分别 50例、37例、20例，采用免疫组织化学法及实时荧光定量 PCR法(RT-PCR)检测各组标本的 FOXO1蛋白及mRNA的表达。结果 FOXO1蛋白在癌性组的染色阳性率为 46.0%，而在良性组和癌旁组中阳性率分别为 75.0%、70.2%；RT-PCR检测结果显示，癌性组、癌旁组、良性组的 FOXO1 mRNA表达量分别为(0.526±0.011)、(0.885±0.017)、(0.841±0.026)，FOXO1蛋白及FOXO1 mRNA在癌性组标本中的表达均较癌旁组及良性组明显降低，差异均有统计学意义(P<0.05)。乳腺癌组织中FOXO1蛋白的表达及FOXO1 mRNA与肿瘤大小、腋窝淋巴结转移有关(P<0.05)，而与患者绝经状态、年龄、人表皮生长因子受体 2 (Her-2)、雌激素受体(ER)、孕激素受体(PR)及Ki-67无关(P>0.05)。结论 FOXO1蛋白及mRNA的表达与乳腺癌肿瘤大小、腋淋巴结转移有关，可作为判断乳腺癌预后的生物学指标之一。
      【关键词】 乳腺癌；FOXO1；免疫组化；实时荧光定量PCR
      【中图分类号】 R737.9 【文献标识码】 A 【文章编号】 1003—6350（2017）09—1383—04

Expression of FOXO1 gene in breast carcinoma and its clinical significance.
CHEN Bao-ying 1, ZHANG Ying 1,HUANG Sheng-chao 2, YAN Ze-ming 2, He-gan 2. Department of Oncology 1, Department of Breast Surgery 2, AffiliatedHospital of Guangdong Medical University, Zhanjiang 524001, Guangdong, CHINA
【Abstract】 Objective To investigate the occurrence and development of FOXO1 gene process and its clinicalsignificance in breast carcinoma. Methods The protein and mRNA expression of FOXO1 gene were detected in 50 cas-es of breast carcinoma, 20 cases of benign breast lesion and 37 cases of adjacent normal breast tissue by an immunohisto-chemical method (steptavidin peroxidase, SP) and fluorescence-based real-time reverse transcription PCR (RT-PCR).These tissue samples were taken from the patients who admitted to the Affiliated Hospital of Guangdong Medical Col-lege from June 2012 to January 2013. Results The positive rate of FOXO1 protein in the cancer group was 46.0%,while the positive rates in the benign group and the adjacent group were respectively 75.0% and 70.2%. The results ofRT-PCR showed that the expression of FOXO1 mRNA in the cancer group, the adjacent cancer group and the benigngroup were (0.526±0.011), (0.885±0.017), (0.841±0.026), respectively; the protein and mRNA expression of FOXO1gene in the cancer group were significantly lower than those in the adjacent cancer group and benign group (P<0.05).The protein and mRNA expression of FOXO1 gene was associated with tumor size, lymph node metastasis (P<0.05), butit had no relationship with menopausal status, age, HER2 receptor protein (HER2), estrogen receptor (ER), progesteronereceptor (PR), Ki67 (P>0.05). Conclusion The protein and mRNA expression of FOXO1 gene is associated with tu-mor size and axillary lymph node metastasis, which means that FOXO1 can be used as one of the biological parametersto determine the prognosis of breast carcinoma.
      【Key words】 Breast carcinoma; FOXO1; Immunohistochemistry; Real-time quantitative PCR·论 著·doi:10.3969/j.issn.1003-6350.2017.09.003