标题：曲格列酮对肿瘤坏死因子α诱导肾小球系膜细胞的作用及其机制

      作者：丁红，王蕾，李慧敏    (中国医科大学附属第四医院肾内科，辽宁 沈阳 110032)

      卷次： 2016年27卷24期

      【摘要】 目的 探讨曲格列酮对肿瘤坏死因子-α (TNF-α)诱导肾小球系膜细胞增殖的影响及机制。方法 将体外培养的大鼠肾小球系膜细胞分为空白组，TNF-α组、TNF-α+曲格列酮组，MTT法检测各组系膜细胞的增殖情况，ELISA法测定各组系膜细胞上清液中细胞间粘附分子1 (ICAM-1)蛋白表达，免疫组化法测定各组系膜细胞核因子κB (NF-κB)的表达情况。结果 TNFα组肾小球系膜细胞 6 h、12 h、24 h和 48 h增殖的OD值分别为(0.198±0.025)、(0.241±0.028)、(0.286±0.030)、(0.267±0.042)，空白组分别为 (0.159±0.021)、(0.161±0.019)、(0.164±0.023)、(0.170±0.020)，TTNF-α+曲格列酮组分别为(0.187±0.023)、(0.184±0.017)、(0.172±0.018)、(0.168±0.026)，TNF-α组肾小球系膜细胞增殖的OD值明显高于空白组，TNF-α+曲格列酮组明显低于TNF-α组，差异均有统计学意义(P<0.05)；TNF-α组肾小球系膜细胞NF-κB蛋白阳性率为(62.34±10.26)%，空白组为(29.97±4.88)%，TNF-α+曲格列酮组为(45.67±8.36)%，TNF-α组肾小球系膜细胞NF-κB蛋白阳性率明显高于空白组，而TNF-α+曲格列酮组则明显低于TNF a组，高于空白组，差异均有统计学意义(P<0.05)；TNF-α组肾小球系膜细胞 ICAM-1蛋白浓度为(967.8±77.4) pg/mL，空白组为(571.2±69.6) pg/mL，TNFα+曲格列酮组为(787.5±81.2) pg/mL，TNF-α组肾小球系膜细胞 ICAM-1蛋白浓度明显高于空白组，TNF-α+曲格列酮组明显低于TNF-α组，但仍高于空白组，差异均有统计学意义(P<0.05)。结论 TNF-α能够促进肾小球系膜细胞增殖，促进NF-κB蛋白和 ICAM-1蛋白表达，曲格列酮能够抑制肾小球系膜细胞增殖，降低NF-κB蛋白和 ICAM-1蛋白表达。
      【关键词】 曲格列酮；肿瘤坏死因子α；肾小球系膜细胞；增殖
      【中图分类号】 R692 【文献标识码】 A 【文章编号】 1003—6350（2016）24—3957—04

Effect of troglitazone on tumor necrosis factor-α induced mesangial cells and its mechanism.
DING Hong, WANGLei, LI Hui-min. Department of Nephrology, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032,Liaoning, CHINA
【Abstract】 Objective To explore the effect of troglitazone on TNF-α induced mesangial cells and its mecha-nism. Methods The cultured mouse glomerular mesangial cells were divided into the blank group, TNF-α group,TNF-α+troglitazone group. The proliferation of mesangial cells was detected by MTT assay. The expression of intercel-lular adhesion molecule-1 (ICAM-1) protein in the supernatants of mesangial cells was determined by enzyme-linkedimmunosorbent assay (ELISA). The expression of nuclear factor-κB (NF-κB) in mesangial cells of different groupswas determined by immunohistochemistry. Results The proliferation OD values of the glomerular mesangial cells inthe TNF-α group at 6 hours, 12 hours, 24 hours and 48 hours were (0.198±0.025), (0.241±0.028), (0.286±0.030), and(0.267 ± 0.042), respectively; the proliferation OD values of the glomerular mesangial cells in the blank group were(0.159±0.021), (0.161±0.019), (0.164±0.023) and (0.170±0.020), respectively; the proliferation OD values of the glo-merular mesangial cells in the TNF-α+ troglitazone group were (0.187 ± 0.023), (0.184 ± 0.017), (0.172 ± 0.018) and(0.168±0.026), respectively. The proliferation OD values of the glomerular mesangial cells of the TNF-α group were sig-nificantly higher than those of the blank group, and the OD values of glomerular mesangial cell proliferation of theTNF-α + troglitazone group were significantly lower than those of the TNF-α group, with statistically significant differ-ences (P<0.05). The positive rate of NF-κB protein in glomerular mesangial cells in the TNF-α group, blank group andTNF-α+ troglitazone group were respectively (62.34±10.26)%, (29.97±4.88)% and (45.67±8.36)%; the positive rate ofNF-κB protein in glomerular mesangial cells in the TNF-α group was significantly higher than that in the blank group;the positive rate of NF-κB protein in glomerular mesangial cells in the TNF-α+troglitazone group was significantlylower than that in TNF-α group and significantly higher than that in blank group, and all of the above differences werestatistically significant (P<0.05). The concentration of ICAM-1 protein in glomerular mesangial cells in the TNF-αgroup, blank group and TNF-α + troglitazone group were respectively (967.8±77.4) pg/mL, (571.2±69.6) pg/mL and(787.5±81.2) pg/mL; the concentration of ICAM-1 protein in glomerular mesangial cells in the TNF-α group was signifi-cantly higher than that in blank group; the concentration of ICAM-1 protein in glomerular mesangial cells in the TNF-α+troglitazone group was significantly lower than that in TNF-α group and significantly higher than that in blank group,·论 著·基金项目：辽宁省科技厅科学技术计划项目(编号：2012225021)