标题：505株金黄色葡萄球菌感染的临床分布及耐药性分析

      作者：胡海岩 1，黄燕妮 2，邬强 2    (1.海南医学院临床技能培训中心，海南 海口 570102；2.海南医学院热带医学与检验医学院，海南 海口 571199)

      卷次： 2016年27卷22期

      【摘要】 目的 了解我院金黄色葡萄球菌感染的临床分布及耐药性特征，为临床正确使用抗生素提供依据。方法 收集并分离2014年1~12月海南医学院附属医院送检得到的505株金黄色葡萄球菌，采用VITEK-32检定仪进行菌种确证，K-B纸片扩散法检测耐药性，使用WHONET5.6软件进行数据分析。结果 505株金黄色葡萄球菌中，临床科室分布构成比前三者为 ICU (12.87%)、儿科(10.89%)和耳鼻喉科(10.89%)；标本主要来自痰(33.66%)；感染以15~64岁的成年人为主；男性(69.31%)构成比高于女性(30.69%)；耐甲氧西林金黄色葡萄球菌(MRSA)检出率为28.71%；未检出利奈唑胺和万古霉素的耐药菌；青霉素耐药率最高，达89.10%。结论 加强耐药菌的监测并合理使用抗生素，对有效控制院内感染具有重要意义。
      【关键词】 金黄色葡萄球菌；感染；分布；耐药性
      【中图分类号】 R378.1+1 【文献标识码】 A 【文章编号】 1003—6350（2016）22—3672—03

Clinical distribution and antibiotic resistance analysis of 505 strains of Staphylococcus aureus.
HU Hai-yan 1,HUANG Yan-ni 2, WU Qiang 2. 1. Clinical Skills Training Center, Hainan Medical University. Haikou 570102, Hainan,CHINA; 2. School of Tropical Medicine and Laboratory Medicine, Hainan Medical University. Haikou 570102, Hainan,CHINA
【Abstract】 Objective To understand the clinical distribution and drug resistance characteristics of Staphylo-coccus aureus, to provide guidance of clinical antibiotics. Methods A total of 505 strains of S. aureus were collectedand separated from Jan 2014 to Dec 2014 in Affiliated Hospital of Hainan Medical University. The isolated strains wereidentified by VITEK32 Automated Microbial Calibrator. The drug susceptibility testing was carried out by K-B diskdiffusion method. The data analysis was performed by WHONET5.6. Results The 505 strains of S. aureus were dis-tributed mainly in ICU (12.87% ), Pediatric Department (10.89% ), E.N.T Department (10.89% ). The samples weremainly from sputum (33.66%). The age of patients infected with S. aureus were mainly 15 to 64 years old. The infec-tion rate of males was higher than that of females (30.69% ). The detection rate of methicillin-resistant S. aureus(MRSA) was 28.71%. The strains were detected not resistant to linezolid and vancomycin, but highly resistant to peni-cillin (89.10% ). Conclusion Strengthening the monitoring of drug-resistant bacteria and using antibiotics rationallywere of great significance to reduce the generation of drug-resistant strains, and to control nosocomial infection.
      【Key words】 Staphylococcus aureus; Infection; Distribution; Drug resistance·论 著·doi:10.3969/j.issn.1003-6350.2016.22.021基金项目：海南省自然科学基金(编号：20158310)