标题：Treg/TH17细胞因子的平衡作用与不同中医症候HBV宫内感染相关性研究

      作者：栗军香 1，王银茹 2，李阿静 3，郝晓丽 4，耿静 5，贾丽霞 6    （1.河北工程大学附属医院检验科，河北 邯郸 056002；2.河北工程大学附属医院妇产科，河北 邯郸 056002；3.邯郸市妇幼保健院功能科，河北 邯郸 056001；4.涉县人民医院妇产科，河北 邯郸 056400；5.河北工程大学附属医院药学部，河北 邯郸 056002；6.河北工程大学医学院生化教研室,河北 邯郸 056002)

      卷次： 2016年27卷21期

      【摘要】 目的 探讨调节性T细胞(Treg)/辅助性T细胞17(Thl7)的平衡作用及其与不同中医症候乙型肝炎病毒(HBV)宫内感染的相关性。方法 选择2014年10月至2015年12月期间在河北工程大学附属医院分娩的单胎足月孕妇及其新生儿各100例，根据是否发生HBV宫内感染分为对照组(未发生HBV，n=50)和观察组(发生HBV，n=50)。观察组孕妇根据不同中医症候分为肝郁脾虚型、湿热中阻型、瘀血阻络型、肝肾阴虚型、脾肾阳虚型5组，应用流式细胞仪检测两组孕妇外周血和新生儿脐血Treg细胞和TH17细胞的表达，应用酶联免疫吸附实验(ELISA)法检测两组Treg和TH17相关细胞因子 IL-10和 IL-17的水平。结果 观察组孕妇和新生儿Treg和TH17细胞的表达及 IL-10和IL-17水平[孕妇分别为(7.92±1.41)%、(5.64±1.61)%、(316.4±32.3) g/mL、(321.6±30.7) g/mL；新生儿分别为(6.53±1.14)%、(5.11±1.35)%、(298.3±35.4) g/mL、(316.7±28.9) g/mL]均明显高于对照组[孕妇分别为(5.87±1.55)%、(3.19±0.47)%、(153.2±31.5) g/mL、(193.4±21.5) g/mL；新生儿分别为(4.02±1.52)%、(2.98±0.38)%、(141.5±26.1) g/mL、(181.6±23.3) g/mL]，差异均有统计学意义(P<0.05)；观察组孕妇和新生儿的 IL-17/IL-10 [(1.01±0.13)、(1.05±0.12)]均小于对照组[(1.31±0.12、1.30±0.11)]，差异均有统计学意义(P<0.05)；观察组5种不同中医症候组间 IL-17/IL-10比较差异有统计学意义(P<0.05)，从高到低依次为肝郁脾虚型、湿热中阻型、瘀血阻络型、肝肾阴虚型、脾肾阳虚型[孕妇分别为(1.25±0.16)、(1.11±0.15)、(0.97±0.14)、(0.83±0.14)、(0.69±0.1)；新生儿分别为(1.22±0.15)、(1.03±0.16)、(0.93±0.15)、(0.81±0.13)、(0.64±0.15)]。结论 发生HBV宫内感染的孕妇和新生儿Treg细胞和TH17细胞及其相关细胞因子的表达均增强，Treg细胞的增幅高于TH17细胞，IL-17/IL-10细胞因子的免疫失衡可能是引起HBV宫内感染的因素之一。
      【关键词】 Treg细胞；TH17细胞；不同中医症候；乙型肝炎病毒；宫内感染
      【中图分类号】 R512.6+2 【文献标识码】 A 【文章编号】 1003—6350（2016）21—3448—04

Relationship between the balance of Treg/TH17 cytokines and the intrauterine infection of HBV in differentTCM syndromes.
LI Jun-xiang 1, WANG Yin-ru 2, LI A-jing 3, HAO Xiao-li 4, GENG Jing 5, JIA Li-xia 6. 1. Departmentof Clinical Laboratory, the Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, CHINA;2. Department of Obstetrics and Gynecology, the Affiliated Hospital of Hebei University of Engineering, Handan 056002,Hebei, CHINA; 3. Department of Function, Handan Maternal and Child Health-Care Hospital, Handan 056001, Hebei,CHINA; 4. Department of Obstetrics and Gynecology, Shexian County People's Hospital, Handan 056400, Hebei, CHINA;5. Department of Pharmacy, the Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, CHINA;6. Department of Biochemistry, Faculty of Medicine of Hebei University of Engineering, Handan 056002, Hebei, CHINA
【Abstract】 Objective To investigate the role of regulatory T cells (Treg) / helper T cell 17 (Thl7) and its corre-lation with the intrauterine infection of hepatitis B virus (HBV) in different traditional Chinese medicines (TCM) syn-dromes. Methods A total of 100 single fetus full-term pregnant women and their newborns coming from AffiliatedHospital of Hebei University of Engineering from October 2010 to December 2015 were divided into the control group(n=50) and observation group (n=50) according to whether the occurrence of HBV intrauterine infection. The observa-tion group was divided into liver and spleen deficiency type, damp heat resistance type, blood stasis type, liver and kid-ney deficiency type, spleen and kidney yang deficiency type 5 groups according to different TCM syndrome. The expres-sion of Treg cells and TH17 cells in peripheral blood of pregnant women and umbilical cord blood of were detected byflow cytometry, and enzyme-linked immunosorbent assay (ELISA) was used to detect the level of cytokines IL-10 andIL-17 that related to TH17 and Treg in the two groups. Results The expression of Treg and TH17 cells and the levelsof IL-10 and IL-17 of pregnant women and newborn in the observation group were (7.92 ± 1.41)% , (5.64 ± 1.61)% ,(316.4±32.3) g/mL, (321.6±30.7) g/mL, (6.53±1.14)%, (5.11±1.35)%, (298.3±35.4) g/mL, (316.7±28.9) g/mL, respec-tively, which were significantly higher than these of pregnant women and newborn in the control group of (5.87±1.55)%,·论 著·doi:10.3969/j.issn.1003-6350.2016.21.003基金项目：河北省邯郸市科技局项目(编号：0823108061-3)