标题：大蒜素联合5-FU对腺样囊性癌ACC-M裸鼠移植瘤的抑制作用

      作者：车超 1，吴发印 1，李华林 1，周鹤峰 2    (1.遵义医学院第五附属珠海医院口腔科，广东 珠海 519100；2.遵义医学院珠海校区生物工程系，广东 珠海 519041)

      卷次： 2016年27卷21期

      【摘要】 目的 探讨大蒜素联合5-FU对腺样囊性癌ACC-M裸鼠移植瘤的抑制作用。方法 建立ACC-M细胞Balb/c裸鼠肩胛下移植瘤模型30只。造模2周后挑选24只肿瘤大小较均匀的裸鼠随机分为实验组(大蒜素组、5-FU组和大蒜素联合5-FU组)和对照组，各6只。实验组按照体质量 0.01 mL/g腹腔注射大蒜素和5-FU浓度均为20 μg/mL，对照组注射0.9% PBS液，每两天一次，共3次，末次注射1周后处死动物。测量移植瘤的体积变化，HE染色观察移植瘤的病理学改变。结果 移植瘤体积变化比较结果：各实验组移植瘤体积明显缩小，5-FU组体积小于大蒜素组，大蒜素联合 5-FU组与大蒜素组、5-FU组比较差异有显著统计学意义(P<0.01)；镜下见对照组的癌细胞生长旺盛，异型率高，而各实验组的肿瘤细胞出现不同程度减少，间质增加，大蒜素联合 5-FU组最为明显。结论 大蒜素和5-FU均能有效抑制ACC-M细胞Balb/c裸鼠移植瘤的生长，且大蒜素联合5-FU的抑制作用明显强于大蒜素或5-FU单独使用。
      【关键词】 大蒜素；5-FU；腺样囊性癌；裸鼠；移植瘤
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2016）21—3441—03

Inhibitory effects of allicin combined with 5-FU on adenoid cystic carcinoma ACC-M in nude mice model.
CHEChao 1, WU Fa-yin 1, LI Hua-lin 1, ZHOU He-feng 2. 1. Department of Stomatology, Zhuhai Hospital, the Fifth AffiliatedHospital of Zunyi Medical University, Zhuhai 519100, Guangdong, CHINA; 2. Department of Bioengineering, ZhuhaiCampus, Zunyi Medical University, Zhuhai 519041, Guangdong, CHINA
【Abstract】 Objective To investigate the inhibitory effect of allicin combined with 5-fluorouracil (5-FU) onadenoid cystic carcinoma in nude mice models transplanted with ACC-M cells. Methods The nude mice xenograftmodels were established by subcutaneous injecting human adenoid cystic carcinoma cell line ACC-M into Balb/c nudemice. Twenty-four xenograft model rats were randomly divided into four groups (control group and three experimentalgroups of allicin group, 5-FU group, and allicin combined with 5-FU group), with 6 rats in each group. The rates in thefour groups received intraperitoneal injection of 0.01 mL/g medicine according to the weight of each nude mice, 0.9%phosphate buffer saline (PBS), 20 μg/mL allicin, 20 μg/mL 5-FU, and 20 μg/mL allicin combined with 20 μg/mL 5-FUrespectively, once per two days, for three times. The animals were executed after 1 week for measuring the xenograft tu-mor volume and observing the pathological changing by HE staining. Results The xenograft tumor volume of three ex-perimental groups had narrowed considerably. The tumor volume was significantly decreased in the 5-FU group com-pared with Allicin group, with statistically significant difference between allicin combined with 5-FU group and allicingroup, 5-FU group (P<0.01). Under microscope, the cancer cells of the control group showed vigorous growth and highabnormal rate, while the cancer cells of the three experimental groups showed reduced quantity and increased intercellu-lar substance to different extent, especially in allicin combined with 5-FU group. Conclusion Allicin and 5-FU canboth effectively inhibit the growth of nude mice xenograft tumor of adenoid cystic carcinoma. And the inhibitory effectsof allicin combined with 5-FU is more significant than either allicin or 5-FU used alone.
      【Key words】 Allicin; 5-fluorouracil; Adenoid cystic carcinoma (ACC); Nude mice; Xenograft tumor·论 著·基金项目：贵州省科学技术基金项目[编号：黔科合J字LKZ(2010)07号]