标题：参松养心胶囊对心梗后心肌的保护作用及机制研究

      作者：刘自强，王 晞，党 松
    (武汉大学人民医院心内科，湖北 武汉 430060)

      卷次： 2015年26卷24期

      【摘要】 目的 探讨参松养心胶囊(SSYX)对心肌梗死后心律失常发生和心功能不全的影响及其作用机
制。方法 将40只新西兰大耳白兔行冠状动脉前降支结扎术后随机分为正常对照组(Control组) 10只、心梗组
(MI组)10只、心梗+胺碘酮组(MI+A组，0.4 g·kg-1·d-1) 10只、心梗+参松组(MI+S组，0.5 g·kg-1·d-1) 10只。正常对
照组开胸后在冠状动脉下穿线，但不结扎。术后，分别以普食、普食+胺碘酮粉末、普食+参松养心胶囊原粉喂
食。8周后将家兔处死，在心梗周边区取材，检测各组白兔缝隙连接蛋白43 (CX43)、钠钙交换体 (NCX)和兰尼碱
受体蛋白(RyR2) mRNA的相对表达量。结果 与Control组比较，MI组CX43、RyR2 mRNA相对表达量显著下
降(P<0.05)，而NCX mRNA相对表达量虽然有所下降，但其差异无统计学意义(P>0.05)；与MI组比较，MI+A组
CX43、RyR2 mRNA相对表达量显著升高(P<0.05)，MI+S组CX43、NCX、RyR2 mRNA的相对表达量也显著升高
(P<0.05)；与MI+A组比较，MI+S组CX43、NCX mRNA的相对表达量均显著升高(P<0.05)。结论 参松养心胶囊
可以改善心肌梗死后心律失常以及心功能不全，其机制可能与调控各钙离子通道的开放，抑制钙超载有关。

      【关键词】 参松养心胶囊；心肌梗死；钙通道；缝隙连接蛋白43；钠钙交换体；兰尼碱受体蛋白

      【中图分类号】 R542.2+2 【文献标识码】 A 【文章编号】 1003—6350（2015）24—3589—04


Myocardial protective effects of shensongyangxin capsule after myocardial infarction and the possible
mechanisms.
LIU Zi-qiang, WANG Xi, DANG Song. Department of Cardiology, Renmin Hospital of Wuhan University,
Wuhan 430060, Hubei, CHINA

【Abstract】 Objective To explore the effect of shensongyangxin capsule (SSYX) on arrhythmia and cardiac
insufficiency after myocardial infarction (MI) in rabbits. Methods Forty New Zealand White rabbits were ran-
domly assigned into control group, MI group, MI+amiodarone group (MI+A group, with the dose of amiodarone at
0.4 g·kg-1·d-1) and MI+SSYX group (MI+S group, with the dose of SSYX at 0.5 g·kg-1·d-1) after successful establish-
ment of model of MI, each with 10 rabbits. The control group applied stitches at coronary artery without ligation. The
other three groups applied routine food, routine food+amiodarone, routine food+SSYX after surgery, respectively. After
eight weeks, ryanodine receptor 2 (RyR2), Connexin43 (CX43), sodium-calcium exchanger (NCX) mRNA expression in
peri-infarcted area were examined. Results Compared with control group, CX43, RyR2 mRNA relative expression sig-
nificantly decreased in MI group (P<0.05), but NCX mRNA relative expression showed slight decrease without statisti-
cally significant difference (P>0.05). Compared with MI group, CX43, RyR2 mRNA relative expression significantly in-
creased in MI+A group (both P<0.05), and CX43, NCX, RyR2 mRNA relative expression significantly increased in MI+
S group (all P<0.05). Compared with MI+A group, CX43, NCX, mRNA relative expression significantly increased in
MI+S group (all P<0.05). Conclusion SSYX can improve arrhythmia and cardiac dysfunction after myocardial infarc-
tion, which may be related to the open of calcium ion channels and the inhibition of calcium overload.

      【Key words】 Shensongyangxin (SSYX); Myocardial infarction (MI); Calcium ion channel; Connexin43
(CX43); Sodium-calcium exchanger (NCX); Ryanodine receptor 2 (RyR2)
基金项目：国家重点基础研究发展计划(973计划)(编号：2012CB518604)