标题：气相色谱法测定甘氨酰胺盐酸盐中氯乙酸甲酯的含量

      作者：李才岛，韦 华
    (海南正瑞医药科技开发有限公司，海南 海口 570311)

      卷次： 2015年26卷22期

      【摘要】 目的 建立测定甘氨酰胺盐酸盐中氯乙酸甲酯含量的气相色谱法。方法 采用气相色谱法。色
谱柱为DB-WAX毛细管柱(固定液：聚乙二醇；规格：30.0 m×0.25 mm×0.25 μm)，柱温为110℃，FID检测器温度为
250℃，进样口温度为200℃，分流比为10:1，顶空瓶平衡温度为85℃，顶空瓶平衡时间为30 min。结果 氯乙酸
甲酯检测浓度的线性范围为 0.008 00~0.128 33 mg/ml (r=0.999 5)；平均回收率为 100.0% (RSD=1.79%)。结论
本方法操作简便快捷，结果准确可靠，可用于甘氨酰胺盐酸盐中氯乙酸甲酯的质量控制。

      【关键词】 甘氨酰胺盐酸盐；氯乙酸甲酯；气相色谱法

      【中图分类号】 R971 【文献标识码】 C 【文章编号】 1003—6350（2015）22—3394—03


Content determination of methyl chloroacetate in glycinamide hydrochloride by gas chromatography.
LI Cai-dao, WEI Hua.
Hainan Zhengrui Medical Technology Development Co., Ltd., Haikou 570311, Hainan, CHINA

【Abstract】 Objective To establish a gas chromatographic method for determining the content of methyl
chloroacetate in glycinamide hydrochloride. Methods Gas chromatography (GC) was adopted with DB-WAX capil-
lary column (Stationary liquid: polyethylene glycol; Specification: 30.0 m×0.25 mm×0.25 μm), column chromatogra-
phy of 110℃, FID detector temperature of 250℃, injection port temperature of 200℃, split ratio of 10:1, equilibrium
temperature of empty bottles of 85℃, and equilibrium time of empty bottles of 30 minutes. Results The linear range
of methyl chloroacetate detected was 0.008 00~0.128 33 mg/ml (r=0.9995). The average recovery rate was 100.0%
(RSD=1.79%). Conclusion This method is fast, easy to operate, accurate and reliable, which can be used in the quali-
ty control of methyl chloroacetate in glycinamide hydrochloride.

      【Key words】 Glycinamide hydrochloride; Methyl chloroacetate; Gas chromatography (GC)