标题：遵义地区2013年手足口病病毒亚型鉴定结果分析

      作者：马晶晶，夏世敏，陈德林，王凤学
    (遵义市第一人民医院检验科，贵州 遵义 563002)

      卷次： 2015年26卷20期

      【摘要】 目的 鉴定遵义地区手足口病病毒亚型，观察其感染病毒类型特征。方法 收集2013年1~12月
期间于遵义市第一人民医院就诊的疑似手足口病例样本，采用细胞分离结合逆转录-聚合酶链反应(RT-PCR)检
测柯萨奇病毒A16型(CA16)、肠道病毒71型(EV71)及肠道病毒通用型(EV)的阳性率，并对所测结果进行统计分
析。结果 ①在 2013年度所有疑似 4 680例手足口病患者的样本检测中阳性者 17 47例，占 37.3%。②在 1 747
例阳性检测标本中年龄小于 2岁者 895例，占所有阳性患者的 51.23%；2~4岁者 659例，占 37.72%；4~6岁者 187
例，占10.70%；大于6岁者6例，占0.34%。③所有手足口病病毒阳性检出患者中CA16型阳性者38例，占2.17%；
EV71型阳性者203例，占11.62%；EV型阳性者1 726例，占98.80%。结论 2013年度遵义地区手足口病主要易
感人群为4岁以下儿童，病毒流行亚型以肠道病毒EV型感染为主。

      【关键词】 手足口病；肠道病毒；亚型；遵义地区

      【中图分类号】 R725.9 【文献标识码】 A 【文章编号】 1003—6350（2015）20—3021—03


Analysis on virus subtypes and epidemiological characteristics of hand-foot-mouth disease in Zunyi in 2013.
MA Jing-jing, XIA Shi-min, CHEN De-lin, WANG Feng-xue.
Department of Clinical Laboratory, the First People's
Hospital of Zunyi, Zunyi 563002, Guizhou, CHINA

【Abstract】 Objective To investigate the virus subtypes of hand-foot-mouth disease (HFMD) in Zunyi, as
well as to analyze the epidemiological features in this area. Methods Samples of suspected HFMD cases in the First
People's Hospital of Zunyi from January to December in 2013 were collected. Cell separation combined with reverse
transcription-polymerase chain reaction (RT-PCR) was used to detect the positive rate of coxsackie virus A16 (CA16),
enterovirus 71 (EV71) and enterovirus (EV). The results was statistically analyzed. Results (1) Among the 4 680 cas-
es suspected of HFMD in 2013, there were 1 747 positive infectious cases, which accounted for 37.3%. (2) Among the
1 747 positive cases, there were 895 cases of less-than-two-year-old children (accounting for 51.23%), 659 cases of 2~
4-year-old children (37.72%), 187 cases of 4~6-year-old children (10.70%), and only 6 cases of more-than-6-year-old
children (0.34%). (3) Among the 1 747 positive cases, there were 38 cases of CA16 subtype (accounting for 2.17%),
203 cases of EV71 subtype (11.62%), and 1 726 cases of Enterovirus (EV) subtype (98.80%). Conclusion The sus-
ceptible population of HFMD in Zunyi in 2013 was the children under the age of 4. The virus subtype was mainly EV.