标题：海南三叉苦抗炎作用及机制研究

      作者：林紫微，赵智萍，林志军，王海龙，任守忠，李佩佩
    （海南医学院药学院，海南 海口 571199）

      卷次： 2016年27卷13期

      【摘要】 目的 研究海南三叉苦水提物的抗炎作用并探索其作用机制。方法 通过二甲苯致小鼠耳肿胀、角
叉菜胶致大鼠足肿胀来观察三叉苦的抗炎作用。采用酶联免疫吸附法(ELISA法)测定肿瘤坏死因子α (TNF-α)和
白细胞介素1β (IL-1β)的含量。结果 与模型组(0)相比，三叉苦水提物中、高剂量(19.11%，22.94%)能抑制二甲苯
所致小鼠耳肿胀(P<0.05)，中剂量对角叉菜胶致大鼠足肿胀有明显的抑制作用，在致炎 4 h [(0.53±0.17) cm]效果
最明显(P<0.01)。水提物中、高剂量对大鼠血清中 TNF-α [(169.61±73.80) ng/L和(124.34±85.65) ng/L]和 IL-1β
[(521.01±206.89) ng/L和(393.99±197.61) ng/L]有不同程度的降低作用，TNF-α较模型组[(283.98±68.68) ng/L]降低
显著(P<0.05)。结论 海南三叉苦水提物具有抗炎作用，其抗炎作用可能与抑制TNF-α和 IL-1β的生成有关。

      【关键词】 三叉苦；抗炎；作用机制；肿瘤坏死因子α；白细胞介素1β

      【中图分类号】 R285.1 【文献标识码】 A 【文章编号】 1003—6350（2016）13—2079—03


Anti-inflammatory effect and mechanism of Evodia lepta in Hainan.
LIN Zi-wei, ZHAO Zhi-ping, LIN Zhi-jun, WANG
Hai-long, REN Shou-zhong, LI Pei-pei. School of Pharmacy, Hainan Medical University, Haikou 571199, Hainan, CHINA

【Abstract】 Objective To study the anti-inflammatory effect and mechanism of Evodia lepta in Hainan.
Methods The anti-inflammatory effect of Evodia lepta was observed by acute inflammation model of the mouse ear
swelling and rat foot swelling. The contents of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β)
were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with the model group, the
middle and high dose water extracts (19.11%, 22.94%, respectively) of Evodia lepta could inhibit the ear swelling in-
duced by xylene (P<0.05); The water extracts at middle doses could remarkably restrained paw edema induced by car-
rageen, with the best anti-inflammatory effect at 4 h [(0.53±0.17) cm, P<0.01]; The water extracts at middle and high
dose could reduce the content of TNF-α [(169.61±73.80) ng/L vs (124.34±85.65) ng/L] and IL-1β [(521.01±206.89)
ng/L vs (393.99±197.61) ng/L] in rat serum; Compared with the model group [(283.98±68.68) ng/L], the TNF-α was
decreased significantly (P<0.05). Conclusion The water extract of Evodia lepta has anti-inflammatory effect, and its
anti-inflammatory effect may be related to inhibiting the formation of TNF-α and IL-1β.

      【Key words】 Evodia lepta (Spreng.) Merr.; Anti-inflammatory; Mechanism; Tumor necrosis factor alpha
(TNF-α); Interleukin 1 beta (IL-1β)
·论 著·
6350.2016.13.005