标题：天麻素对大鼠离体胸主动脉血管环的作用及机制研究

      作者：钟节雄，杨宏华
    (武汉市商业职工医院心血管内科，湖北 武汉 430000)

      卷次： 2016年27卷13期

      【摘要】 目的 观察天麻素对大鼠离体胸主动脉环的作用，并探讨其作用机制。方法 采用离体动脉环灌流方法，
观察累加浓度天麻素对大鼠离体胸主动脉环的直接作用；同法观察不同浓度天麻素(5 μmol/L、50 μmol/L、100 μmol/L、
150 μmol/L、200 μmol/L、250 μmol/L)对10-6 mol/L去氧肾上腺素(PE)预收缩有内皮和去内皮血管环的作用；用无Ca2+
K-H液和不同浓度天麻素孵育对PE收缩去内皮血管环的作用；观察200 μmol/L天麻素对60 mmol/L KCl预收缩血管环
的作用。结果 天麻素直接作用于血管环时，天麻素组与对照组血管紧张度比较差异无统计学意义(P>0.05)；天麻素(≥
50 μmol/L)作用于PE预收缩血管环时，有内皮天麻素组血管环舒张率分别高于有内皮对照组[(41.9±8.9）%、(57.6±9.1)%、
(65.0±10.2)%、(75.6±11.7)%、(81.9±12.0)% vs (10.5±1.6)%、(19.4±5.9)%、(22.6±7.2)%、(24.9±6.8)%、(25.1±7.3)%、P<
0.05]，去内皮天麻素组血管环舒张率分别高于去内皮对照组[(20.6±2.9)%、(36.5±8.2)%、(43.7±9.3)%、(55.7±9.6)%、
(58.7±9.6)% vs (9.8±2.1)%、(15.8±4.7)%、(18.6±6.5)%、(20.3±5.0)%、(21.5±5.1)%，P<0.05]，且均有浓度依赖性，多组间比
较F值分别为5.67，5.99，6.11，6.47，7.05，P<0.05；天麻素作用于无钙K-H液中PE收缩去内皮血管环时，实验组舒张率
[(5.1±1.2)%]小于对照组[(12.8±3.7)%]，差异有统计学意义(t=5.599，P<0.05)。天麻素处理作用于60 mmol/L KCl预收缩
的血管环时，天麻素组[（96.7±8.6）%]与对照组[(98.6±7.9)%]张力变化差异无统计学意义(t=0.581，P>0.05)。结论 天麻
素对正常血管无舒张作用；天麻素能舒张PE预收缩的血管，其机制是通过抑制血管平滑肌 IP3受体介导的内钙释
放；它不能抑制α1受体依赖性钙通道和电压依赖性钙通道；天麻素舒张PE收缩的血管的作用有内皮依赖性和浓度
剂量依赖效应。

      【关键词】 大鼠；天麻素；胸主动脉环；作用；机制

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2016）13—2073—04


Effect and mechanism of gastrodin on isolated thoracic aorta rings in rats.
ZHONG Jie-xiong, YANG Hong-hua.
Department of Cardiovascular Medicine, Wuhan Commercial Staff Hospital, Wuhan 430021, Hubei, CHINA

【Abstract】 Objective To observe the effect and mechanism of gastrodin on isolated thoracic aorta rings in rats.
Methods A perfusion model of isolated thoracic aorta ring in rats was applied to observe the direct effect of cumulative
gastrodin on isolated thoracic aorta rings in rats. The same method was also used to explore the effects of the different
concentration gastrodin (5 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L, 200 μmol/L, 250 μmol/L) on endothelium-in-
tact/denuded aorta rings pre-contracted with 10-6 mol/L phenylephrine (PE). PE was used to contract the aorta rings incu-
bated by 200 mmol/L gastrodin in the Ca2 + free K-H solution, and the effect of 200 mmol/L gastrodin on aorta rings
pre-contracted with 60 mmol/L KCl was observed. Results With the direct effect of gastrodin on the endothelium-in-
tact vascular ring, there was no significant difference between the gastrodin group and the control group (P>0.05) on the
vascular tension. With the effect of cumulative concentration of gastrodin (≥50 μmol/L) on PE pre-contracted vascular
ring were studied, the vascular ring relaxation rate in endothelium-intact gastrodin group and endothelium-denuded gas-
trodin group were significantly higher than control group [(41.9±8.9)%, (57.6±9.1)%, (65.0±10.2)%, (75.6±11.7)%,
(81.9±12.0)% vs (10.5±1.6)%, (19.4±5.9)%, (22.6±7.2)%, (24.9±6.8)%, (25.1±7.3)%, P<0.05]; Moreover, the rate of
relaxation of vascular rings in the endothelium-intact gastrodin group were higher than in the endothelium-denuded
gastrodin group [(20.6±2.9)%, (36.5±8.2)%, (43.7±9.3)%, (55.7±9.6)%, (58.7±9.6)%) vs (9.8±2.1)%, (15.8±4.7)%,
·论 著·
6350.2016.13.003