标题：615例门诊就诊者HPV基因检测结果分析

      作者：林丽华，钟 娜，乔 凤，张丽芬
    (海南省皮肤性病防治中心，海南 海口 570206)

      卷次： 2015年26卷18期

      【摘要】 目的 了解海口地区人乳头瘤病毒(HPV)感染的情况，为HPV感染患者的治疗及监测提供科学依
据。方法 采用实时荧光定量 PCR方法(FQ-PCR)对海口地区 615例性病门诊就诊者泌尿系分泌物标本的
HPV6，11型和HR-HPV (8个型)进行检测。 结果 615例样本中检出HPV阳性者 302例，感染率为 49.11%
(302/615)，其中HPV6，11-DNA阳性 130例，阳性率为 21.14% (130/615)，HR-HPV-DNA阳性 126例，阳性率为
20.49% (126/615)，HPV6，11型合并HR-HPV阳性 46例，占 7.48% (46/615)。HPV6，11低危型与HR-HPV混合高
危型比较差异无统计学意义(P>0.05)。302例HPV感染者中尖锐湿疣(CA)患者187例，占61.92% (187/302)，检出
HPV6,11-DNA阳性 126例，占 67.38% (126/187)，HR-HPV-DNA阳性 19例，占 10.16% (19/187)，HPV6,11型和
HR-HPV混合感染42例，占22.46% (42/187)。CA患者感染以HPV6，11低危型为主，HPV6，11/HR-HPV次之，少
部分为高危混合型HR-HPV感染，各组比较差异有统计学意义(P<0.01)。结论 尖锐湿疣主要的基因型感染是
低危型HPV6,11，高危型HPV感染多以混合感染形式存在。荧光定量PCR方法检测尖锐湿疣患者HPV基因型
具有快速、准确的优点，是临床HPV感染分型检测的有效方法。

      【关键词】 人乳头瘤病毒；FQ-PCR；感染率；尖锐湿疣

      【中图分类号】 R373 【文献标识码】 A 【文章编号】 1003—6350（2015）18—2723—02


Human papillomavirus gene analysis of 615 out-patients.
LIN Li-hua, ZHONG Na, QIAO Feng, ZHANG Li-fen.
Hainan Provincial Center for STD/Skin Disease Control and Prevention, Haikou 570206, Hainan, CHINA

【Abstract】 Objective To explore human papillomavirus (HPV) infection in people in Haikou, China, and
provide a basis for the treatment and monitoring of HPV-infected patients. Methods The urinary secretion speci-
mens of 615 patients from Out-patient Department of Venereal Diseases were analyzed with real-time fluorescent
quantitation PCR (FQ-PCR) for HPV6,11 and HR-HPV (8 types). Results Of the 615 specimens, 302 (49.11%, 302/
615) were positive of HPV, including 130 (21.14%, 130/302) positive of HPV6,11 DNA, 126 (20.49%, 126/302) posi-
tive of HR-HPV DNA, and 46 (7.48%, 46/302) positive of both HPV6,11 and HR-HPV DNA. There was no statistical-
ly significant difference between the low-risk group (HPV6,11) and the high-risk group (HPV6,11 combined with
HR-HPV), P>0.05. Of the 302 HPV-infected patients, 187 (61.92%, 187/302) had condyloma acuminata (CA), out of
which 126 (67.38%, 126/187) were positive for HPV6,11 DNA, 19 (10.16%, 19/187) positive for HR-HPV DNA and
42 (22.46% , 42/187) positive for both HPV6,11 and HR-HPV DNA. Infection with low-risk HPV6,11 was mostly
seen in these CA patients, followed by mixed infection with both HPV6,11 and HR-HPV and then by high-risk infec-
tion with HR-HPV, where the differences among groups were significant (P<0.01). Conclusion The main genotype
of CA is low-risk HPV6,11, while the high-risk genotype of HPV often exists in a mixed status. Being rapid and pre-
cise in the determination of HPV genotype in CA patients, fluorescent quantitation PCR is an effective clinical method
for classifying HPV infection.

      【Key words】 Human papillomavivus; Fluorescent quantitation PCR; Infection rate; Condyloma acuminata