标题：荧光定量PCR检测女性生殖道解脲脲原体原体的应用评价

      作者：黄 会 1，赵香依 2，吴多荣 1，姚 敏 1
    (1.中南大学湘雅医学院附属海口医院检验科，海南 海口 570208；
2.乐东县人民医院检验科，海南 乐东 572500)

      卷次： 2015年26卷18期

      【摘要】 目的 了解荧光定量PCR检测解脲脲原体（Uu）敏感性、特异性及其临床应用价值。方法 收集海
口人民市医院 2013年 3~5月妇产科门诊泌尿生殖道感染患者生殖道标本共 154例，同时进行荧光定量PCR和
液-固体培养法检测Uu，以液-固体培养法作为金标准，对荧光定量PCR诊断试验进行方法学评价。结果 154
例标本中，液-固体培养法即金标准检测阳性 64例，荧光定量PCR检测阳性 69例，两者同时阳性 63例，同时阴
性 84例，荧光定量PCR检测Uu敏感性是 98.44% (63/64)，特异性是 93.33% (84/90)，假阳性率是 6.67% (6/84)，假
阴性率是 1.56% (1/64)，阳性预测值是 91.30% (63/69)，阴性预测值是 98.82% (84/85)，总符合率是 95.45%
(147/154)。χ2检验显示，两种方法检测结果差异无统计学意义(P>0.05)，计算Kappa>0.75，表明两种方法检测结果
的一致性程度较好。结论 荧光定量PCR方法具有灵敏度高、特异好、检测快速的优点，且与培养法一致性也较
高，具有一定的临床应用价值。

      【关键词】 荧光定量PCR；解脲脲原体；应用

      【中图分类号】 R711.3 【文献标识码】 A 【文章编号】 1003—6350（2015）18—2720—03


Application of fluorescence quantitative PCR for detection of Ureaplasma urealyticum in female genital tract.
HUANG Hui 1, ZHAO Xiang-yi 2, WU Duo-rong 1, YAO Ming 1.
1. Department of Clinical Laboratory, Haikou Hospital
Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan, CHINA; 2. Department of
Clinical Laboratory, People's Hospital of Ledong County, Ledong 572500, Hainan, CHINA

【Abstract】 Objective To evaluate the sensitivity, specificity, and application of fluorescence quantitative
PCR (FQ-PCR) for detection of Ureaplasma urealyticum (Uu) in female genital tract. Methods A total of 154 speci-
mens of patients with genital tract infections were detected by FQ-PCR and liquid-solid culture at the same time. All
specimens were collected in Haikou People's Hospital from March 2013 to May 2013. Results In the 154 specimens,
64 and 69 samples were revealed as positive according to the golden standard method and FQ-PCR method respective-
ly, with 63 samples positive and 84 negative for both the golden standard method and FQ-PCR method. For FQ-PCR,
the sensitivity rate was 98.44% (63/64), and the specificity rate was 93.33% (84/90), with the false positive rate of
6.67% (6/84), the false negative rate of 1.56% (1/64), positive predictive value of 91.30% (63/69), negative predictive
value of 98.82% (84/85), and the total coincidence rate of 95.45% (147/154). χ2 test results showed that there was no
·论 著·doi:10.3969/j.issn.1003-6350.2015.18.0987