标题：前列腺素E2在川崎病患者血浆中的表达及临床意义

      作者：杨燕澜，武宇辉，黄雀兰，李成荣，王国兵，马伟科
    (深圳市儿童医院内二科，广东 深圳 518026)

      卷次： 2015年26卷18期

      【摘要】 目的 观察前列腺素E2 (PGE2)在川崎病(KD)患儿血浆中的表达水平并探讨其临床意义。方法 选择
2013年5月至2014年10月在我院住院的35例KD急性期患儿，在静脉注射免疫球蛋白(IVIG)治疗前后采用双抗体
夹心酶联免疫吸附试验(ELISA)测定血浆PGE2水平，实时荧光定量PCR法测定外周血CD14+单核/巨噬细胞(MC)环
氧化酶-1 (COX-1)、环氧化酶-2 (COX-2)、肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6)水平。另选15例健康同
龄儿童作为对照组，测定上述指标。结果 KD急性期患儿血浆PGE2、COX-1、COX-2、TNF-α、IL-6水平均高于对
照组，差异均有统计学意义(P<0.05)；KD患儿中的冠状动脉病变组PGE2、COX-2水平高于无冠状动脉病变组，差异
均有统计学意义(P<0.05)，但 COX-1、TNF-α、IL-6比较则差异均无统计学意义(P>0.05)；IVIG治疗后 PGE2、
COX-1、COX-2、TNF-α、IL-6水平较治疗前均降低，差异均有统计学意义(P<0.05)。结论 KD急性期患儿血浆
PGE2水平升高，其可能在KD的炎症免疫反应和氧化应激中发挥重要作用。

      【关键词】 川崎病；前列腺素E2；环氧化酶-2

      【中图分类号】 R442.8 【文献标识码】 A 【文章编号】 1003—6350（2015）18—2675—04


Changes and significance for prostaglandin E2 level in plasma of patients with Kawasaki disease.
YANG Yan-lan,
WU Yu-hui, HUANG Que-lan, LI Cheng-rong, WANG Guo-bing, MA Wei-ke. The Second Department of Medicine,
Shenzhen Children's Hospital, Shenzhen 518026, Guangdong, CHINA

【Abstract】 Objective To observe the prostaglandin E2 (PGE2) level in plasma of patients with Kawasaki dis-
ease (KD) and discuss the clinical significance. Methods Thirty-five patients with KD which treated in our hospital
from May 2013 to October 2014 were selected. The plasma concentrations of PGE2 were analyzed by ELISA tech-
nique. Real-time quantitative PCR was used to evaluate the expression level of cyclooxygenases-1 (COX-1), cycloox-
ygenases-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) on CD14 + monocyte/macrophage cells
(MC) before and after intravenous immunoglobulin (IVIG). Fifteen healthy children were selected as the control
group, with the above indicators measured. Results The levels of PGE2, COX-1, COX-2, TNF-α and IL-6 in acute
KD patients were significantly higher than those in the control group (P<0.05), and they were down-regulated after
IVIG (P<0.05). The level of PGE2 and COX-2 in KD patients with coronary artery lesion were significantly higher
than those without coronary artery lesion (P<0.05), and there were no statistically significant difference in COX-1,
TNF-α and IL-6 between the two groups (P>0.05). Conclusion Prostaglandin E2 level in plasma of patients with
KD is up-regulated and may play an important role in inflammatory and immune response and oxidative stress.