标题：BGC-823胃癌细胞凋亡相关蛋白受miR-421调控后的表达分析

      作者：浦雄勇，姚永良，孙王伟，蒋一彪，吴建红
    (江苏大学附属昆山医院检验科，江苏 昆山 215300)

      卷次： 2015年26卷17期

      【摘要】 目的 研究miR-421在胃癌BGC-823细胞凋亡过程中的分子机制。方法 通过siRNA转染技术将
miR-421 mimics与miR-421 inhibitors转染 BGC-823胃癌细胞；Western blotting方法分析细胞凋亡相关蛋白
caspase-3、Bax、Bcl-2以及细胞凋亡受体蛋白 TNFR-Ⅰ、TNFR-Ⅱ的表达水平。结果 miR-421 mimics与
miR-421 inhibitors成功转染BGC-823胃癌细胞；caspase-3、Bax蛋白在miR-421 inhibitors转染细胞中表达增强，而
Bcl-2、TNFR-Ⅰ与 TNFR-Ⅱ则表达降低。结论 miR-421在BGC-823胃癌细胞中可能通过调控肿瘤细胞凋亡相
关蛋白caspase-3、Bax、Bcl-2的表达及肿瘤细胞表面凋亡受体蛋白TNFR的表达从而影响BGC-823的凋亡。

      【关键词】 胃癌；miR-421；转染

      【中图分类号】 R735.2 【文献标识码】 A 【文章编号】 1003—6350（2015）17—2500—03


Expression analysis of related proteins in the apoptosis of BGC-823 gastric cancer cells regulated by miR-421.
PU Xiong-yong, YAO Yong-liang, SUN Wang-wei, JIANG Yi-biao, WU Jian-hong.
Department of Clinical Laboratory,
Kunshan People's Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu, CHINA

【Abstract】 Objective To study the molecular mechanism of miR-421 in the apoptosis of BGC-823 gas-
tric cancer cells. Methods miR-421 mimics and miR-421 inhibitors were transfected into BGC-823 cells by
siRNA transfection technology. The expression of proteins related to apoptosis or receptors such as caspase-3,
Bax, Bcl-2, TNFR-Ⅰ and TNFR-Ⅱwere detected by Western blotting. Results The miR-421 mimics and miR-421
inhibitors were transfected into BGC-823 cells successfully. The expression of caspase-3 and Bax proteins were en-
hanced in BGC-823 cells transfected with miR-421 inhibitors, while the expression of Bcl-2, TNFR-Ⅰ and TNFR-
Ⅱ were decreased. Conclusion The expression of apoptosis proteins caspase-3, Bax, Bcl-2 and cell surface recep-
tor proteins TNFR are regulated by miRNA-421 to influence the apoptosis of BGCe-823 gastric cancer cells.

      【Key words】 Gastric cancer; miR-421; Transfection