标题：兔短阵室性心动过速心肌病模型的建立

      作者：曾向辉 1，周小华 1，孙 翔 1，凌 静 1，卿艳云 1，张 翼 2
    (1.长沙市中医医院(长沙市第八医院)心内科，湖南 长沙 410100；
2.湖南师范大学第一附属医院心内科，湖南 长沙 410005)

      卷次： 2015年26卷16期

      【摘要】 目的 探讨建立兔短阵室性心动过速心肌病模型方法。方法 15只雄性新西兰兔。在无X线、微
创、静脉下用标测电极行短阵室速刺激右心室建立心动过速性心肌病动物模型。用10极冠状窦电极，经改良5F
桡动脉鞘，经右侧前腔静脉送至右心室，连接程序刺激仪，起搏右心室。结果 起搏1周后心脏彩超检查证实左
心室舒张末内径扩大，左心室射血分数降低，血清B型脑利钠肽(BNP)、基质金属蛋白酶9 (MMP-9)升高、金属蛋
白酶抑制剂1 (TIMP-1)下降。HE染色显示心肌结构排列紊乱，细胞间质炎性浸润。电镜下显示线粒体肿胀，甚
至溶解、破坏，Z线模糊，闰盘结构显示不清。3周后再复查心脏彩超、血清BNP、MMP-9、TIMP-1，心脏射血分数
(EF)、短轴缩短率(FS)均大致恢复起搏前水平。结论 无X线、微创静脉下用标测电极行短阵室速刺激右心室建
立心动过速性心肌病动物模型简单可行。

      【关键词】 心动过速性心肌病；短阵室性心动过速；B型脑钠肽；兔；模型

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2015）16—2347—04


Exploration of a method to establish rabbit models of nonsustained ventricular tachycardia cardiomyopathy.
ZENG Xiang-hui 1, ZHOU Xiao-hua 1, SUN Xiang 1, LING Jing 1, QING Yan-yun 1, ZHANG Yi 2.
1. The Hospital of
Traditional Chinese Medicine of Changsha (the Eighth Hospital of Changsha), Changsha 410100, Hunan, CHINA; 2.
Department of Cardiology, the First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan, CHINA

【Abstract】 Objective To explore a effective method to establish rabbit models of nonsustained ventricular
tachycardia cardiomyopathy. Methods Fifteen New Zealand rabbits were used in this study. The right ventricle was
stimulated with mapping electrode line to create nonsustained ventricular tachycardia by minimally invasive venous
technique without X-ray radiation. A 10 polar cap of sinus electrode was inserted through modified 5F radial artery
sheath and the right anterior vena cava to the right ventricle. Then the programming stimulator was connected to pace
the right ventricle to create the tachycardia cardiomyopathy animal model. Results After one week of right ventricular
pacing, cardiac ultrasound examination showed enlarged left ventricular end diastolic diameter, reduced left ventricular
ejection fraction, increased serum B-type natriuretic peptide (BNP) and matrix metalloproteinase 9 (MMP-9), and de-
creased tissue inhibitor of metallopmteinase-1 (TIMP-1). HE staining showed myocardial structural derangement and
·论 著·doi:10.3969/j.issn.1003-6350.2015.16.0848