标题：二氢杨梅素-镍配合物体内外抗白假丝酵母菌作用研究

      作者：杨 文 1，麦明晓 2，李海霞 3，王 英 1，周贞鉴 1，李文广 1
    （1.海南医学院热带医学与检验医学院，海南 海口 571101；
2.海南医学院理学院，海南 海口 571101；
3.海南医学院药学院，海南 海口 571101）

      卷次： 2015年26卷16期

      【摘要】 目的 观察二氢杨梅素-镍配合物(DMY-Ni)体外对白假丝酵母菌的抗菌活性，探讨DMY-Ni治
疗小鼠白假丝酵母菌阴道炎的疗效。方法 采用试管二倍稀释法测定DMY-Ni对临床 30例白假丝酵母菌
(海南医学院附属医院门诊患者阴道分泌物中分离)的最低抑菌浓度(MIC)值和最低杀菌浓度(MBC)值，同时
模拟小鼠白假丝酵母菌阴道炎模型，分组给药，SPSS17.0单因素方差分析法和配对 t检验进行统计学分析。
结果 DMY-Ni对白假丝酵母菌具有较强的抗菌活性，MIC为 6.25~50 ?g/ml，MBC为 12.5~200 ?g/ml，分别于
模型组和给药前比较，4种剂量组给药 7 d后小鼠阴道内白假丝酵母菌落数均明显下降（(P<0.05或P<0.01)，阴
道局部病变肉眼观察逐渐好转，且高剂量组的治疗效果最佳。结论 4种剂量DMY-Ni对白假丝酵母菌阴道
炎模型小鼠都有明显治疗效果，高剂量组的治疗效果要优于另外3种剂量组，为获得临床治疗白假丝酵母菌阴
道炎提供一个可参考的途径。

      【关键词】 二氢杨梅素-镍配合物；白假丝酵母菌；抗菌活性

      【中图分类号】 R711.34 【文献标识码】 A 【文章编号】 1003—6350（2015）16—2341—04


Study on the anti-Candida albicans acitivity of Dihydromyricetin-Nickel complex in vivo and in vitro.
YANG
Wen 1, MAI Ming-xiao 2, LI Hai-xia 3, WANG Ying 1, ZHOU Zhen-jian 1, LI Wen-guang 1. 1. School of Tropical and
Laboratory Medicine, Hainan Medical University, Haikou 571101, Hainan, CHINA; 2. School of Basic Medicine, Hainan
Medical University, Haikou 571101, Hainan, CHINA; 3. College of Pharmacy, Hainan Medical University, Haikou
571101, Hainan, CHINA

【Abstract】 Objective To study the anti-Candida albicans acitivity of Dihydromyricetin-Nickel complex
(DMY-Ni complex) in vitro, and to explore the efficacy of DMY-Ni complex for treating mice model of vulvovaginitis
candidiasis (VVC). Methods Tube double dilution method is used to determine the minimum inhibitory concentra-
tions (MICs) and minimum bactericidal concentrations (MBCs) value of DMY-Ni complex to Candida albicans (sam-
ples were isolated from the vaginal secretions of 30 out-patients in the Affiliated Hospital of Hainan Medical Col-
lege ). At the same time, we established mice model of VVC, and DMY-Ni complex as vaginal drug delivery was as-
signed to observe the efficacy for VVC mice model. The data were analyzed using statistical software SPSS17.0 sin-
gle factor analysis of variance and t test comparative analysis. Results DMY-Ni complex has strong activity in vi-
tro against Candida albicans with MIC ranging from 6.25 ?g/ml to 50 ?g/ml and MBC ranging from 12.5 ?g/ml to
200 ?g/ml. Compared with the mice model of VVC, four kinds of DMY-Ni complex concentration could significantly
reduce the colony number of Candida albicans in vagina (P<0.05 or P<0.01). Vaginal local symptoms gradually im-
proved, and high dose group had the best therapeutic effect. Conclusion Four doses DMY-Ni complex all have sig-
nificant therapeutic effect in the VVC mice, and the effect of high dose group is superior to that of other three dose
groups. The results can provide reference for the treatment of VVC.

      【Key words】 Dihydromyricetin-Nickel complex; Candida albicans; Antimicrobial activity
基金项目：海南医学院科研培育基金（编号：2014-014）