标题：Periostin在肾细胞癌中的表达及其临床意义

      作者：江伟东，朱 黎，廖国龙，曾志宇，刘志龙
    (珠海市第二人民医院泌尿外科，广东 珠海 519020)

      卷次： 2015年26卷11期

      【摘要】 目的 研究骨细胞特异性因子2 (Periostin)在肾细胞癌组织中的表达，探讨其与肾细胞癌临床病理
因素和预后的关系及意义。方法 应用Western blot和Real-time PCR检测 90例肾细胞癌组织及相对应的癌旁
肾脏组织中Periostin表达情况，并与临床病理资料及预后进行相关性分析。结果 76.67% (69/90)的肾癌组织的
Periostin表达高于相对应的癌旁组织，65.56% (59/90)肾癌组织的Periostin mRNA表达高于相对应的癌旁组织，
肾细胞癌组织Periostin高表达与肿瘤大小、肾盂侵犯、癌栓、淋巴转移相关(P<0.05)，肾细胞癌组织Periostin高表
达的患者术后生存时间显著短于Periostin低表达者(P<0.01)。结论 Periostin表达升高与肾细胞癌的增殖、转
移、浸润与生存时间密切相关，Periostin可作为预测肾细胞癌转移和判断预后的指标。

      【关键词】 肾细胞癌；Periostin；转移；预后

      【中图分类号】 R737.11 【文献标识码】 A 【文章编号】 1003—6350（2015）11—1578—03


Expression and clinical significance of Periostin in renal cell carcinoma.
JIANG Wei-dong, ZHU Li, LIAO
Guo-long, ZENG Zhi-yu, LIU Zhi-long. Department of Urology, the Second People's Hospital of Zhuhai, Zhuhai
519020, Guangdong, CHINA

【Abstract】 Objective To evaluate the mRNA and preotein levels of Periostin in renal cell carcinoma (RCC),
and to analyze the relationships between its protein level and the clinical pathological features, prognosis of RCC.
Methods Western blot and real-time PCR were applied to detect the expression levels of Periostin in 90 cases of
RCC and the adjacent tissues. The correlations between expression of Periostin and pathological features, prognosis
were analyzed. Results 76.67% (69/90) and 65.56% (59/90) patients showed significant higher protein and mRNA lev-
els in RCC than adjacent tissues, respectively. The abnormal expression of Periostin correlated with tumor size, renal pel-
vis invasion, tumor thrombus and lymph metastasis (P<0.05). Patients with elevated expression of Periostin in RCC had
significantly shorter postoperative survival time than those with low expression of Periostin (P<0.01). Conclusion The
high expression of Periostin correlates with proliferation, invasion, metastasis and patient prognosis. Our results suggest
that Periostin may be a potential marker for determining the degree of malignancy and prognosis.

      【Key words】 Renal cell carcinoma; Periostin; Metastasis; Prognosis
基金项目：广东省中医药局课题立项（编号：20131049）