标题:丝裂霉素、吡柔比星和免疫调节剂联合灌注对膀胱癌患者血清及冲洗液中粘附分子、蛋白酶表达的影响
作者:杨 渝,朱晓应,郭嘉祥
(中国人民解放军总医院第一附属医院泌尿外科,北京 100048)
卷次:
2015年26卷10期
【摘要】 目的 分析丝裂霉素、吡柔比星和免疫调节剂联合灌注对膀胱癌患者血清及冲洗液中粘附分子、
蛋白酶表达的影响。方法 选取2012年10月至2014年10月间在本院接受经尿道膀胱肿瘤电切术(TURBT)治
疗的非肌性浸润性膀胱癌患者86例,其中接受丝裂霉素、吡柔比星膀胱灌注治疗者(对照组)和接受丝裂霉素、吡
柔比星和免疫调节剂联合灌注治疗者(观察组)各43例。检测两组患者治疗后的血清及冲洗液中的粘附分子、蛋
白酶表达情况。结果 (1)血清粘附分子:观察组血清细胞粘附分子(CD44v6)、E-钙黏素(E-cadherin)、肝细胞粘
附分子(hepaCAM)水平高于对照组,上皮特异性粘附分子(EpCAM)、可溶性细胞间粘附分子-1 (sICAM-1)、可溶
性血管黏附分子-1 (sVCAM-1)水平低于对照组,差异均有统计学意义(P<0.05);(2)冲洗液粘附分子:观察组冲洗
液CD44v6、E-cadherin、hepaCAM水平高于对照组,EpCAM、sICAM-1、sVCAM-1水平低于对照组,差异均有统
计学意义(P<0.05);(3)蛋白酶:观察组血清及冲洗液中基质金属蛋白酶2 (MMP2)、基质金属蛋白酶9 (MMP9)水
平低于对照组,半胱氨酸蛋白酶-8 (Caspase-8)水平高于对照组,差异均有统计学意义(P<0.05)。结论 丝裂霉
素、吡柔比星和免疫调节剂联合灌注可以调节膀胱癌患者的粘附分子及蛋白酶表达,抑制侵袭转移能力、促进肿
瘤细胞凋亡。
【关键词】 膀胱癌;免疫调节剂;粘附分子;蛋白酶
【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350(2015)10—1427—04
Effect of combined perfusion of mitomycin, pirarubicin, immunomodulators on the expression of adhesion
molecule, protease in serum and rinses of patients with bladder cancer.
YANG Yu, ZHU Xiao-ying, GUO
Jia-xiang. Department of Urology, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, CHINA
【Abstract】 Objective To analyze the effect of combined perfusion of mitomycin, pirarubicin, immuno-
modulators on the expression of adhesion molecule, protease in serum and rinses of patients with bladder cancer.
Methods Eighty-six patients of non-muscle-invasive bladder cancer received transurethral resection of bladder tu-
mor (TURBT) in our hospital from October 2012 to October 2014 were selected as study objects, including 43 cases
of the control group with mitomycin and pirarubicin for perfusion treatment and 43 cases of the observation group
with mitomycin, pirarubicin combined with immunomodulators for perfusion treatment. The expression of adhesion
molecules, protease in serum and rinses were detected after treatment. Results (1) In serum, CD44v6, E-cadherin,
hepaCAM levels in the observation group were significantly higher than those in the control group, while EpCAM, sI-
CAM-1, sVCAM 1 levels in the observation group were significantly lower. (2) In rinses, CD44v6, E-cadherin,
hepaCAM levels in the observation group were significantly higher than those in the control group, while EpCAM, sI-
CAM-1, sVCAM-1 levels in the observation group were significantly lower. (3) For protease, MMP-2, MMP-9 lev-
els in serum and rinses of the observation group were significantly lower than those in the control group, while Cas-
pase-8 level of the observation group was significantly higher. Conclusion Combined perfusion of mitomycin, pira-
rubicin immunomodulators can adjust the expression of adhesion molecules and protease in patients with bladder can-
cer, inhibit invasion and metastasis ability, and promote apoptosis of tumor cells.
下载PDF