标题：金雀异黄素抑制肝癌干细胞样细胞的特性及对氟尿嘧啶耐药性的影响

      作者：李 翀，唐纯娜，贺更生
    (南华大学附属第一医院普外科，湖南 衡阳 421001)

      卷次： 2015年26卷8期

      【摘要】 目的 研究金雀异黄素对 SMMC-7721细胞系肝癌干细胞样细胞特性以及氟尿嘧啶敏感性的
影响。方法 不同浓度金雀异黄素处理 SMMC-7721细胞系球形成细胞。肿瘤球形成法检测自我更新能
力。MTT比色法测定氟尿嘧啶药物敏感性。Western blot分析干细胞标志物CD133、CD44、Gli1和ABCG2蛋白
表达。结果 金雀异黄素显著抑制SMMC-7721细胞肿瘤球形成，并优先抑制球形成细胞增殖活性(P<0.05)。
金雀异黄素(10 μmol/L)有效逆转球形成细胞对氟尿嘧啶的耐药性。金雀异黄素降低CD133、CD44、Gli1和ABCG2
蛋白表达水平。结论 金雀异黄素抑制肝癌干细胞样细胞特性和氟尿嘧啶耐药性，其作用机制与抑制干细胞标
志物蛋白表达和阻断Hedgehog信号传导有关。

      【关键词】 肝细胞癌；肿瘤干细胞；金雀异黄素；多药耐药

      【中图分类号】 R735.7 【文献标识码】 A 【文章编号】 1003—6350（2015）08—1108—04


Inhibition of genistein on characteristics of liver cancer stem-like cells and its effect on fluorouracil-resistance.
LI Chong, TANG Chun-na, HE Geng-sheng.
Department of General Surgery, the First Affiliated Hospital, University of
South China, Hengyang 421001, Hunan, CHINA

【Abstract】 Objective To investigate the effects of genistein on the characteristics of liver cancer stem-like
cells (LCSLCs) derived from SMMC-7721 cell line and the sensitivity to fluorouracil. Methods Sphere-forming
cells (SFCs) from SMMC-7721 cell line were treated with various concentrations of genistein. The capability of
self-renewal was tested using tumor sphere-forming assay. The sensitivity to fluorouracil was determined by MTT as-
say. And the protein expression of stem cell markers CD133, CD44, Hedgehog (Hh) signal effector Gli1 and its target
molecule ABCG2 was analyzed using Western blot. Results Genistein significantly inhibited the capability of
sphere-forming in SMMC-7721 SFCs and relative selectively suppressed the proliferation activity of SFCs (P<0.05).
Genistein (10 μ mol/L) could effectively reverse the resistance of SFCs to fluorouracil. And genistein could also
down-regulate the protein expression of CD133, CD44, Gli1 and ABCG2. Conclusion Genistein inhibits characteristics
of liver cancer stem cells and resistance to Fluorouracil, and its mechanism is likely involved in inhibiting protein ex-
pression of stem cell markers and obstructing Hh signal pathway.

      【Key words】 Liver cancer; Cancer stem cells; Genistein; Multidrug resistance