标题：胎鼠肢芽软骨染色技术的一点改进

      作者：夏卉芳 1，2
    （1.贵州师范学院，贵州 贵阳 550018；
2.贵州特色生物资源开发利用重点实验室，贵州 贵阳 550018）

      卷次： 2015年26卷6期

      【摘要】 目的 改进阿利新蓝单染胎鼠肢芽软骨技术，尤其是改进后的技术免用对人体有毒或致癌的试
剂。方法 将SPF级SD大鼠13~16 d龄的胚鼠前或后肢芽用阿利新蓝染液染色、丙三醇透明，最后将透明好的
肢芽标本用DPX树脂镶嵌在载玻片上待观测。结果 胎鼠前肢芽各部分发育分化的软骨均被染成蓝色，标本完
全符合在体视显微镜下对肢芽软骨是否畸形的观测及前肢芽软骨半定量Neubert 300分评价标准。结论 阿利
新蓝单染胎鼠肢芽软骨技术经改进后，肢芽软骨染色好，实验步骤简化、时间缩短、节约成本、安全环保。

      【关键词】 胚胎；肢芽；软骨染色；阿利新蓝

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2015）06—0785—02


Some improvements to the staining of limb bud cartilage in fetal mouse.
XIA Hui-fang 1, 2. 1. Guizhou Normal
College, Guiyang 550018, Guizhou, CHINA; 2. Key Laboratory of Guizhou Characteristics Biological Resources
Development and Utilization, Guiyang 550018, Guizhou, CHINA

【Abstract】 Objective To improve the simple Alcian blue staining of limb bud cartilage in fetal mouse using no
toxic or carcinogenic reagents. Methods The forelimb buds or hindlimb buds of SPF SD rats (13~16 days old) were
stained by Alcian blueand then immersed in glycerol. The transparent specimens were placed onto the slide by DPX resin
for observation. Results All differential cartilage of forelimb buds were dyed blue. And all the specimens met the criteria
of deformity observations under stereo microscope and the semi-quantitative Neubert 300 evaluation criteria of the fore-
limb buds cartilage. Conclusion The improved Alcian blue simple staining technique could help enhance staining effect,
simplify experimental procedure, shorten staining time and cut cost, and it was safe and environmental-friendly.