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      标题:BBG染色兔眼内界膜效果分析
      作者:王 虎,袁建枚,全婵娟,杨新怀
    (中山市小榄人民医院眼科,广东 中山 528415)
      卷次: 2015年26卷4期
      【摘要】 目的 探讨不同染色时间及不同染色剂浓度的亮蓝G (Brilliant blue G,BBG)对内界膜染色的效果
差异。方法 用 12只新西兰白兔按照随机化原则分为四组,行常规 23G玻璃体切割术,气液交换后 1组给予
0.125 mg/ml BBG染色 15 s,2组给予 0.125 mg/ml BBG染色 120 s,3组给予 0.25 mg/ml BBG染色 15 s,4组给予
0.25 mg/ml BBG染色120 s,染色后BSS冲洗干净,手术显微镜下观察染色效果。结果 1组完全染色1眼,不完
全染色 1眼,未见染色 4眼;2组完全染色 5眼,不完全染色 1眼;3组完全染色 1眼,不完全染色 3眼,未见染色 2
眼;4组完全染色5眼,不完全染色1眼。染色结果四组间差异均具有显著统计学意义(P=0.008),根据平均秩次推
断第 2组与第 4组染色效果好于第 3组,第 1组染色效果最差。结论 BBG染色兔眼内界膜效果与染色时间相
关,时间越长,染色效果越好,与染色剂浓度相关性不高。

      【关键词】 BBG;内界膜;兔

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350(2015)04—0478—03


Dyeing effect of brilliant blue G on internal limiting membrane in rabbits.

WANG Hu, YUAN Jian-mei, QUAN
Chan-juan, YANG Xin-huai. Department of Ophthalmology, Xiaolan People's Hospital of Zhongshan, Zhongshan
528415, Guangdong, CHINA

【Abstract】 Objective To analyze the dyeing effect of brilliant blue G (BBG) on internal limiting membrane
(ILM) at different time and different concentration. Methods Conventional 23-G pars plana vitrectomy was per-
formed in the eyes of 12 New Zealand white rabbits and the experiment eyes were randomized into four groups. After
Gas-fluid exchanged, four groups were given different concentrations of BBG solution to dye the ILM for different
time: group 1 (0.125 mg/ml BBG for 15 s); group 2 (0.125 mg/ml BBG for 120 s); group 3 (0.125 mg/ml BBG for 15 s);
group 4 (0.125 mg/ml BBG for 120 s). Then BBG was cleaned up by BSS. The results were observed by the opera-
tion microscope. Results Group 1: complete dyeing 1 eye; incomplete dyeing 1 eye; not dyeing 4 eyes. Group 2:
基金项目:广东省医学科学技术研究基金(编号:A829)

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