标题：芬维A胺对人肝癌细胞株HepG2增殖与细胞凋亡的影响

      作者：石胜利，戴小华，马娅梅，周海华
    (中山小榄人民医院消化内科，广东 中山 528415)

      卷次： 2015年26卷4期

      【摘要】 目的 探讨分子靶向药物芬维 A胺(Fenretinide)在体外对人肝癌细胞株 HepG2的细胞增殖
与细胞周期的影响。方法 体外培养人肝癌 HepG2 细胞，实验组在培养液中加入不同浓度的芬维 A胺
(2.5 μmol/L、5 μmol/L、10 μmol/L、20 μmol/L)，对照组培养液中不加芬维A胺，分别孵育48 h后，采用MTT法检
测芬维A胺对HepG2细胞增殖的影响，流式细胞仪分析细胞周期和凋亡率。结果 芬维A胺能明显抑制HepG2
细胞增殖，呈时间和剂量依赖性；芬维A胺处理HepG2细胞72 h，可使G0~G1期细胞比例升高，G2~M、S期比例明
显下降，凋亡率明显上升(P<0.05)。结论 芬维A胺对体外人肝癌HepG2细胞具有明显的抑制作用，主要表现为
抑制增殖和促进凋亡。

      【关键词】 芬维A胺；人肝癌细胞株HepG2；细胞增殖；凋亡

      【中图分类号】 R735.7 【文献标识码】 A 【文章编号】 1003—6350（2013）04—0472—03


Effect of fenretinide on the proliferation and apoptosis of human liver cancer cell line hepG2.
SHI Sheng-li, DAI
Xiao-hua, MA Ya-mei, ZHOU Hai-hua. Department of Gastroenterology, Zhongshan Xiaolan People's Hospital,
Zhongshan 528415, Guangdong, CHINA

【Abstract】 Objective To investigate the depressant effects of fenretinide on human liver cancer cell line
HepG2 cells in vitro. Methods Human liver carcinoma line HepG2 cells were cultured in vitro. The HepG2 cells of the
test group were incubated in the medium with fenretinide at different concentrations (2.5 μmol/L, 5 μmol/L, 10 μmol/L,
20 μmol/L). The cells of the control group were incubated in the medium for 48 hours. MTT method was used to de-
tect the antiproliferative ratio of fenretinide on HepG2 cells, and flow cytometry was applied to analyze the cell cycle
and apoptotic ratio. Results Fenretinide could obviously inhibit the proliferation of HepG2 cells, showing time- and
dose-dependent effects. 72 hours after the HepG2 cells were treated with fenretinide, the percentage of HepG2 cells in
G0~G1 period increased, and that in G2~M period and S period decreased, with the aopototic ratio of HepG2 cells in-
creased significantly (P<0.05)．Conclusion Fenretinide can inhibit proliferation and promote apoptosis of human
liver cancer cell line HepG2 cells in vitro．

      【Key words】 Fenretinide; Human liver cancer cell line HepG2; Cell proliferation; Apoptosis
基金项目：2012中山市科技局立项课题(编号：20122A106)