标题：血清低密度脂蛋白胆固醇Friedwald计算法与均相法测定结果比较

      作者：吕 杨 1，董 矜 2
    (1.中国人民解放军第187中心医院检验科，海南 海口 571159；
2.中国人民解放军总医院生化科，北京 100853)

      卷次： 2015年26卷1期

      【摘要】 目的 探讨低密度脂蛋白胆固醇 (LDL-C) Friedewald计算法 (F法)与均相法的结果相关性。
方法 调取进行血脂检测[包括总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋
白胆固醇(LDL-C)]的984例空腹标本的检测数据，按TG水平分组比较F法与均相法所得LDL-C的相关性及其对
血脂水平风险评估的影响。结果 两法所得LDL-C的相关性均良好(R≥0.817)，两法所得LDL-C的差异无统计
学意义(P>0.05)。随着TG升高，两种方法血脂水平分级的一致性降低。结论 F法计算LDL-C适用于多数人群
(无乳糜，TG<4.52 mmol/L)血脂水平的评价；F法的准确使用需严格排除Ⅲ型及继发性高脂蛋白血症。

      【关键词】 低密度脂蛋白胆固醇；Friedewald计算法；均相法；血脂

      【中图分类号】 R446 【文献标识码】 A 【文章编号】 1003—6350（2015）01—0064—04


Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay
and by the Friedewald formula.
LV Yang 1, DONG Jin 2. 1. Department of Clinical Laboratory, the 187th Hospital of
Chinese PLA, Haikou 571159, Hainan, CHINA; 2. Department of Biochemistry, the PLA General Hospital, Beijing 100853,
CHINA

【Abstract】 Objective To compare the low-density lipoprotein cholesterol (LDL-C) concentrations measured
by a direct homogeneous assay and by the Friedewald formula. Methods A total of 984 routine samples with triglyc-
eride (TG) concentrations <4.52 mmol/L were enrolled for blood lipids examination, including total cholesterol (TC),
triglyceride (TG), high density lipoprotein cholesterol (HDL-C), LDL-C. LDL-C were measured by both enzymatic
homogeneous assay (LDL-C(D)) and Friedewald formula (LDL-C(F)). Correlation and linear regression analysis were
performed, and the concordance of the National Cholesterol Education Program (NCEP) risk category was estimated
by paired-samples t test. Results Correlation between LDL-C(D) and LDL-C(F) was quite good (R≥0.817). The dif-
ference between LDL-C(F) and LDL-C(D) was not statistically significant (P>0.05). The concordance between LDL-C
(F) and LDL-C (D) declined as TG increased. Conclusion Friedewald formula is valid for the estimation of most
TG<4.52 mmol/L specimen without chyle。 Patients with type Ⅲ or secondary hyperlipoproteinemia should be
screened to ensure the accuracy of Friedewald method.

      【Key words】 Low-density lipoprotein cholesterol (LDL-C); Friedewald formula; Homogeneous assay; Blood
lipid