标题：FHIT基因和蛋白在鼻咽癌组织及正常鼻咽组织中的表达

      作者：罗海清，黄 静，杨东红，陈梓宏，余忠华
    （广东医学院附属医院肿瘤中心二区，广东 湛江 524001）

      卷次： 2014年25卷24期

      【摘要】 目的 研究FHIT基因和蛋白在鼻咽癌组织以及正常鼻咽组织中的表达及其临床意义。方法 采
用实时荧光定量PCR (RQ-RT-PCR)方法和免疫组织化学染色法分析检测54例鼻咽癌组织和28例正常鼻咽组织
FHIT基因和蛋白表达水平。结果 鼻咽癌组织中FHIT基因和蛋白的表达显著低于正常鼻咽组织（P<0.050），且
FHIT基因和蛋白的表达与病理分化程度、临床分期密切相关(P<0.05)，与患者的年龄、性别、癌组织的浸润深度
无关(P>0.05)。结论 FHIT基因和蛋白可能有助于鼻咽癌的诊断，它可作为鼻咽癌的预后因子。

      【关键词】 FHIT；鼻咽癌；基因；蛋白；表达

      【中图分类号】 R739.63 【文献标识码】 A 【文章编号】 1003—6350（2014）24—3640—03


Expression of FHIT gene and its protein in nasopharyngeal carcinoma tissues and normal nasopharyngeal
tissues.
LUO Hai-qing, HUANG Jing, YANG Dong-hong, CHEN Zi-hong, YU Zhong-hua. Oncology Center, Affiliated
Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, CHINA

【Abstract】 Objective To investigate the expression of FHIT gene and its protein in nasopharyngeal carcino-
ma tissues and normal nasopharyngeal tissues, and explore its clinical significance. Methods The expression of
FHIT gene and its protein was assessed by RQ-RT-PCR and immunohistochemical staining in 54 nasopharyngeal car-
cinoma and 28 normal nasopharyngeal tissues. Results The expression of FHIT gene was significantly lower in naso-
pharyngeal carcinoma tissues than that in normal nasopharyngeal tissues (P<0.05). The expression of FHIT gene and
its protein was closely related to differentiation and clinical stage (P<0.05) and not related to age, gender and tumor in-
vasion depth (P>0.05). Conclusion FHIT gene and its protein may contribute to clinical diagnosis of nasopharyn-
geal carcinoma, and it may be a prognostic factor for nasopharyngeal carcinoma.

      【Key words】 FHIT; Nasopharyngeal cancer; Gene; Protein; Expression