标题：α-硫辛酸对实验性变态反应性

      作者：脑脊髓炎大鼠脊髓组织 IL-17表达的影响
    徐晓臣 1，赵冉冉 2，徐 飞 3，李彦改 1，陈朝旺 2，檀国军 4
(1.邯郸市第一医院神经内科，河北 邯郸 056002；
2.邯郸市第一医院急诊科，河北 邯郸 056002；
3.邯郸市第一医院泌尿科，河北 邯郸 056002；
4.河北医科大学第二医院神经内科，河北 石家庄 050071)

      卷次： 2014年25卷24期

      【摘要】 目的 研究α-硫辛酸 (Alpha-lipoic acid，ALA)对实验性变态反应性脑脊髓炎 (Experimental
autoimmune encephalomyelitis，EAE)大鼠模型中脊髓组织 IL-17表达的影响。方法 健康雌性Wistar大鼠50只，
随机分为正常对照组 (NC组)，EAE 7 d组 (EAE-7 d组)、EAE 6个月病程组 (EAE-6 m组)、硫辛酸急性期组
(ALA-7 d组)和硫辛酸6个月病程组(ALA-6 m组)。HE染色观察病理学改变，免疫组织化学染色观察 IL-17的表
达变化。结果 HE染色结果显示，在α-硫辛酸治疗急性期组和6个月病程组，均可减轻脊髓组织的炎性反应和
髓鞘损伤。免疫细胞化学染色结果显示，EAE-7 d组大鼠 IL-17蛋白的表达比正常对照组增加(P<0.01)；EAE-6 m
组大鼠 IL-17蛋白的表达比正常对照组增加(P<0.01)；α-硫辛酸处理后急性期组和6个月病程组 IL-17蛋白的表
达明显降低(P<0.01)。结论 α-硫辛酸在EAE大鼠模型中的保护作用可能是通过抑制 IL-17的表达来实现的。

      【关键词】 多发性硬化；α-硫辛酸；炎症；IL-17

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2014）24—3589—04


Effect of alpha-lipoic acid on IL-17 expression in rats with experimental allergic encephalomyelitis.
XU
Xiao-chen 1, ZHAO Ran-ran 2, XU Fei 3, LI Yan-gai 1, CHEN Chao-wang 2, TAN Guo-jun 4. 1. Department of Neurology,
the First Hospital of Handan, Handan 056002, Hebei, CHINA; 2. Department of Emergency, the First Hospital of Handan,
Handan 056002, Hebei, CHINA; 3. Department of Urology, the First Hospital of Handan, Handan 056002, Hebei, CHINA;
4. Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050071, Hebei, CHINA

【Abstract】 Objective To explore the effects of alpha-lipoic acid (ALA) on IL-17 expression in rats with ex-
perimental allergic encephalomyelitis (EAE). Methods Fifty female Wistar rats were divided randomly into five
groups: the normal control group (NC group), EAE model group with 7 days (EAE-7 d group), EAE model group
with 6 months (EAE-6 m group), ALA administrated EAE model group with 7 days (ALA treated EAE-7 d group),
ALA administrated EAE group with 6 months (ALA treated EAE-6 m group). HE staining was used to detect the
pathologic changes. Immunohistochemistry was used to detect the expression of IL-17. Results HE staining showed
that, in alpha-lipoic acid treated groups (ALA treated EAE-7 d group, ALA treated EAE-6 m group), inflammatory reac-
tion and myelin damage were all relieved. According to immunohistochemistry, the expression of IL-17 in EAE-7 d
group and in EAE-6 m group was significantly increased compared to NC group (P<0.01). However, the expression of
IL-17 in alpha-lipoic acid treated groups significantly decreased compared with EAE-7 d group and EAE-6 m group re-
spectively (P<0.01). Conclusion Alpha-lipoic acid may exert its neuroprotective effect through ameliorating inflamma-
tive reacts.

      【Key words】 Mutiple sclerosis; Alpha-lipoic acid; Inflammation; IL-17
基金项目：河北省科技厅课题（编号：11276425）