标题：CDH1基因甲基化状态与结直肠癌临床病理特征及预后的相关性研究

      作者：方日，王小文
    (湖南师范大学附属湘南医院中国人民解放军第169医院肿瘤科，湖南 衡阳 421002)

      卷次： 2016年27卷11期

      【摘要】 目的 探讨CDH1基因启动子所在5'-CpG岛的异常甲基化与临床病情发展、病理变化及术后预后的
相关性。方法 选取2013年1~12月在我院肿瘤科进行结直肠癌切除术的原发性结直肠癌患者184例，其中甲基化
组86例，非甲基化组98例，所有入选患者由专人进行跟踪随访。检测CDH1基因启动子所在5'-CpG岛的异常甲基
化，通过全基因组扩增技术对修饰后的DNA进行扩增，根据GenBank基因库设计CDH1基因甲基化特异性引物及
CDH1基因非甲基化特异性引物。结果 甲基化组肿瘤直径为(6.5±2.1) cm，大于非甲基化组的(3.2±2.2) cm，甲基
化组肿瘤浸润性所占比例54.7%，高于非甲基化组的42.9%，差异均有显著统计学意义(P<0.01)；甲基化组肿瘤分化
程度(高分化 14.0%，中分化 36.0%)低于非甲基化组(高分化 40.8%，中分化 45.9%%)，差异有显著统计学意义(P<
0.01)；甲基化组淋巴转移率为67.4%、远处转移率为31.4%，高于非甲基化组的21.4%和17.3%，差异均有统计学意
义(P<0.05)；甲基化组临床TNM分期更晚(甲基化组：Ⅰ期11.6%，Ⅱ期11.6%，Ⅲ期37.2%，Ⅳ期39.5%；非甲基化组：
Ⅰ期 27.6%，Ⅱ期 32.7%，Ⅲ期 20.4%，Ⅳ期 19.4%)，差异均有显著统计学意义(P<0.01)；非甲基化组患者生存率
(89.5%)高于甲基化组患者(68.7%)，差异有统计学意义(P<0.05)。Cox比例风险模型结果显示，腹腔灌洗液悬浮细
胞CDH1基因甲基化是原发性结直肠癌患者术后预后生存率的独立危险因素(RR=28.5，P<0.01)。结论 原发性结
直肠癌患者腹腔灌洗液悬浮细胞CDH1基因甲基化程度提高，恶性程度高，预后差。

      【关键词】 结直肠癌；CDH1基因；甲基化；腹腔灌洗液；预后

      【中图分类号】 R735 【文献标识码】 A 【文章编号】 1003—6350(2016)11—1779—04


Correlation between CDH1 gene methylation status and clinical pathological characteristics and prognosis of
colorectal cancer.
FANG Ri, WANG Xiao-wen. Department of Oncology, the 169th Hospital of People's Liberation Army,
Xiangnan Hospital Affiliated to Hunan Normal University, Hengyang 421002, Hunan, CHINA

【Abstract】 Objective To investigate the correlation between the abnormal methylation of 5'-CpG islands har-
boring cadherin 1 (CDH1) gene promoter in and the development of clinical condition, pathological changes and postop-
erative prognosis. Methods A total of 184 patients with colorectal cancer resection of primary colorectal cancer in De-
partment of Oncology in our hospital were selected and divided into methylation group (n=86) and non-methylation
group (n=98) from January to December 2013. All patients were selected and followed up by specially-assigned person.
The abnormal methylation of 5'-CpG islands harboring cadherin 1 (CDH1) gene promoter was detected, and modified
DNA genome was amplified by whole genome amplification technology. CDH1 gene methylation specific primers and
CDH1 gene non-methylation specific primers were designed according to GenBank. Results The diameter of methyla-
tion group was significantly larger than that of non-methylation group [(6.5±2.1) cm vs (3.2±2.2) cm], and the proportion
·论 著·
6350.2016.11.018