标题：螺旋藻多糖对阿尔茨海默病模型小鼠脑线粒体氧化应激保护

      作者：林智君 1，陈煜森 1，郑选梅 2，尹小健 1，刘洲 1，贾真 1，赵江浩 1，赵斌 1
    (1.广东医学院附属医院神经内科，广东 湛江 524001；
2.岭南师范学院体育科学学院，广东 湛江 524048)

      卷次： 2016年27卷11期

      【摘要】 目的 探索螺旋藻多糖对D-半乳糖联合亚硝酸钠诱导的阿尔茨海默病(AD)模型小鼠脑线粒体损伤
的抗氧化保护作用。方法 小鼠腹腔注射D-半乳糖120 mg/kg和亚硝酸钠100 mg/kg制备AD模型。将40只小鼠
按数字编号随机分为正常组(C组 )、模型组(M组)以及3个剂量实验组，每组8只，3个剂量实验组分别给予螺旋藻
多糖灌胃低剂量(El组) 6.7 mg·kg-1·d-1，中剂量(Em组) 67.0 mg·kg-1·d-1，高剂量(Eh组) 134.0 mg·kg-1·d-1，连续8周，
在给药第8周后，进行Morris水迷宫实验和生化指标测试。结果 与M组相比，Em组、Eh组120 S内跨越平台的次
数和在目标象限的探索时间均明显增加(P<0.05)；小鼠脑部β淀粉样蛋白(Aβ1~42)含量减少，依次是M组>El组>Em组
>C组>Eh组。氧化应激指标：与M组相比，锰超氧化物歧化酶（Mn-SOD）活性均有提高(P<0.05)，依次是C组
(64.22±22.31) Um/mg>Eh组(58.36±21.30) Um/mg>Em组(56.58±15.66) Um/mg>E1组(55.32±21.25) Um/mg>M组
(42.68±11.58) Um/mg；活性氧(NOS)含量Eh组(30.70± 4.68) mmol/L显著下降(P<0.05)，丙二醛(MDA)活性Em、Eh
组[(35.55±7.97) nmol/mg、(30.51±8.74) nmol/mg]显著下降；与E1组相比，Eh组抗氧化效果最好。结论 螺旋藻多
糖对阿尔茨海默病模型小鼠脑线粒体氧化应激有保护作用，是脑线粒体营养素，高剂量效果最佳。

      【关键词】 脑线粒体；螺旋藻多糖；阿尔茨海默病；氧化应激

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2016）11—1731—03


Protective effect of spirulina polysaccharide against cerebral mitochondrial oxidative stress in Alzheimer's
disease mice model.
LIN Zhi-jun 1, CHEN Yu-sen 1, ZHENG Xuan-mei 2, YIN Xiao-jian 1, LIU Zhou 1, JIA Zhen 1, ZHAO
Jiang-hao 1, ZHAO Bin 1. 1. Department of Neurology, the Affiliated Hospital of Guangdong Medical College, Zhanjiang
524001, Guangdong, CHINA; 2. College of Physical Education, Lingnan Normal University, Zhanjiang 524048, Guangdong,
CHINA

【Abstract】 Objective To study the protective effect of spirulina polysaccharide against cerebral mitochondrial
oxidative stress induced by D-galactose and sodium nitrite in Alzheimer's disease (AD) mice model. Methods A total of
40 mice were randomly divided into the normal group (C), model group (M), and other three experimental groups (spirulina
polysaccharide, El group of 6.7 mg·kg-1·d-1, Em group of 67.0 mg·kg-1·d-1, and Eh group of 134.0 mg·kg-1·d-1, 8 weeks),
with 8 mice in each group. The model group and three experimental groups were injected with D-galactose 120 mg/kg and
sodium nitrite 100 mg/kg for AD model. Morris water maze (MWM) experiment and Biochemical index test were per-
formed after 8 weeks. Results Compared with group M, the number of crossed platform in 120 s and target exploration
time in Em group and Eh group were significantly increased (P<0.05); While the Aβ1~42 content in brain were decreased
with the order: M>El>Em >C>Eh. Compared with group M, the activity of manganese superoxide dismutase (MnSOD)
were improved with the order: C [(64.22±22.31) Um/mg] >Eh [(58.36±21.30) Um/mg]>Em [(56.58±15.66) Um/mg]>
E1 [(55.32±21.25) Um/mg] >M [(42.68±11.58) Um/mg]. The activities of reactive oxygen species (ROS) in Eh group
[30.70±4.68 mmol/L] and Malondialdehyde (MDA) in Em group [(35.55±7.97) nmol/mg] and Eh group [(30.51±8.74) nmol/mg]
were significantly decreased (P<0.05). Compared with El group, Eh group had the best protective effect against oxida-
tive stress. Conclusion Spirulina polysaccharide can protect AD model mice from the damage of cerebral mitochondri-
al oxidative stress, which is the nutrients of cerebral mitochondria, and high dose of spirulina polysaccharide has the best
protective effect.

      【Key words】 Cerebral mitochondria; Spirulina polysaccharide; Alzheimer's disease; Oxidative stress
·论 著·
6350.2016.11.003