标题：肠源性高草酸尿大鼠模型的建立

      作者：陈清江 1，李 逊 1，雷 鸣 2，何永忠 1
    (1.广州医科大学附属第五医院泌尿外科，广东 广州 510700；
2.广州医科大学附属第一医院海印微创外科中心，广东 广州 510120)

      卷次： 2014年25卷14期

      【摘要】 目的 建立肠源性高草酸尿的大鼠模型。方法 随机选取30只雌性SD大鼠置于大鼠代谢笼中适
应性喂养7 d之后收集24 h尿，记录尿量，采用离子色谱仪测定其草酸浓度，计算正常大鼠在该条件下24 h尿草
酸排泄量。菠菜汁灌胃处理大鼠，再测定灌胃后24 h尿草酸排泄量。对比灌胃菠菜汁前后，大鼠24 h尿草酸排
泄量的差别；分组对照实验，随机选取20只雌性SD大鼠，随机分成A组和B组，B组灌胃菠菜汁，A组灌胃等量纯
净水，其他条件相同，比较两组24 h尿草酸排泄量。结果 反复测定，正常大鼠灌胃菠菜汁前24 h尿草酸排泄量
为(0.53±0.18) mg，灌胃菠菜汁后第1天为(1.93±0.36) mg，第3天为(1.85±0.38) mg，第7天为(2.03±0.39) mg；A组
(0.44±0.12) mg，B组(1.68±0.38) mg。结论 大鼠在灌胃菠菜汁之后其尿液24 h草酸排泄量明显增加，大概增加
3~4倍，说明每天对大鼠进行定量定浓度的菠菜汁灌胃，可以使其保持长期的高草酸尿状态，该方法建立的肠源
性高草酸尿大鼠模型简便可行，可广泛用于其他方面的研究。

      【关键词】 肠源性高草酸尿；尿；大鼠模型；离子相色谱

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2014）14—2036—03


Establishment of rats model with enterogenous hyperoxaluria.
CHEN Qing-jiang 1, LI Xun 1, LEI Ming 2, HE
Yong-zhong 1. 1. Department of Urology Surgery, the Fifth Affiliated Hospital of Guangzhou Medicine University,
Guangzhou 510700, Ghuangdong, CHINA; 2. Haiyin Minimally Invasive Surgery, the First Affiliated Hospital of
Guangzhou Medicine University, Guangzhou 510120, Ghuangdong, CHINA

【Abstract】 Objective To establish enterogenous hyperoxaluria model in rats. Methods Thirty randomly
selected female Sprague-Daw-ley rats were fed in individual metabolic cage for 7 days. 24 h urine oxalate excretions
were examined by ion chromotagraphy before and after the spinach juice lavage, and the difference was investigated.
Another 20 female Sprague-Daw-ley rats were randomly divided into group A and group B. Group B was lavaged
with spinach juice and group A was lavaged with equivalent pure water. 24 h urine oxalate excretions of group A and
group B were compared. Results The 24 h urine oxalate excretion was (0.53±0.18) mg before lavaging spinach juice. On
first day, third day and seventh day after lavaging spinach juice, the 24 h urine oxalate excretions were (1.93±0.36) mg,
(1.85±0.38) mg, and (2.03±0.39) mg，respectively. The 24 h urine oxalate excretion of group A and group B were
(0.44±0.12) mg and (1.68±0.38) mg, respectively. Conclusion The 24 h urine oxalate excretion of SD rats was mark-
edly increased 3 to 4 times after lavaged with spinach juice. Daily lavage with quantitatively and constant concentra-
tion spinach juice can maintain long-term high oxalic acid urine state in rats. This method of establishing enterogenous
hyperoxaluria model in rats is simple and feasible, which can be widely used.

      【Key words】 Enterogenous hyperoxaluria; Urine; Rats model; Ion chromotagraphy
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