标题：胸腺肽α1对维持性血液透析患者外周血T淋巴细胞亚群、NK细胞及 IL-2水平的影响

      作者：孟洁，覃学勇
    (南方医科大学附属柳州医院肾内科，广西 柳州 545007)

      卷次： 2016年27卷10期

      【摘要】 目的 探讨使用胸腺肽α1对糖尿病终末期肾病维持性血液透析(血透)患者外周血T淋巴细胞亚群、
自然杀伤(NK)细胞及白细胞介素-2 (IL-2)水平的影响。方法 选取2011年7月至 2015年6月在我院血透中心行
规律维持性血透患者71例，使用随机数表法完全随机化分为A组36例和B组35例，A组加用胸腺肽α1皮下注射，
每次1.6 mg，每周2次，共用4周；B组不使用胸腺肽α1；C组40例为健康体检者。应用流式细胞术分别检测试验第
一天和一个月时A、B两组外周血T淋巴细胞亚群、NK细胞水平，同时ELISA法检测其外周血血清 IL-2水平。C组
各指标则均为第一天检测。结果 糖尿病终末期肾病维持性血透患者细胞免疫功能及 IL-2水平较正常人显著降
低，两组比较差异有统计学意义(P<0.05)；A组患者治疗后的外周血CD3+、CD4+、CD4+/ CD8+比值及NK细胞明显高于
治疗前[CD3+(%)：(51.51±9.22) vs (72.51±10.22)；CD4+(%)：(26.85±4.21) vs (37.15±6.21)；CD4+/CD8+：(0.70±0.11) vs
(1.63±0.23)；NK细胞(%)：(26.56±6.82) vs (36.21±5.32)]，而CD8+水平明显低于治疗前[(38.24±7.54)% vs (24.26±5.51)%，
外周血血清 IL-2水平明显升高[(15.83±1.82) pg/mL vs (46.23±1.16) pg/mL]，差异均有统计学意义（P<0.05）；B组患
者治疗前后的T淋巴细胞亚群、NK细胞及血清 IL-2水平比较差异均无统计学意义(P>0.05)。结论 胸腺肽α1可提
高糖尿病终末期肾病维持性血透患者细胞免疫功能，可能对其感染性疾病的防治有一定临床意义。

      【关键词】 胸腺肽α1；2型糖尿病；血液透析；免疫功能

      【中图分类号】 R459.5 【文献标识码】 A 【文章编号】 1003—6350（2016）10—1599—03


Impact of thymosin α 1 on cellular immune function and serum IL-2 of patients with maintained hemodialysis.
MENG Jie, QIN Xue-yong.
Department of Nephrology, the Affiliated Liuzhou Hospital of Southern Medical University,
Liuzhou 545007, Guangxi, CHINA

【Abstract】 Objective To observe the impact of thymosin α 1 on cellular immune function and serum IL-2 of
patients with maintained hemodialysis. Methods A total of 71 cases of patients with end-stage renal disease, who un-
derwent maintained hemodialysis in our hospital from July 2011 to June 2015, were randomly divided into group A (36
cases with 1 cycle of treatment of thymosin α1) and group B (35 cases without treatment of thymosin α1), and 40 cases of
healthy persons were chosen as the group C. Group A appiled thymosin α1 (subcutaneous injection, 1.6 mg each time, 2
times a week, a total of 4 weeks), while group B didn't. Peripheral blood T lymphocyte subsets and NK cells were detect-
ed by flow cytometry at the first day and after one month of treatment. Serum IL-2 was identified by ELISA (enzyme
linked immune-sorbent assay). Results The cellular immune function and IL-2 level in patients with maintained hemo-
dialysis were significantly lower than the healthy people (P<0.05). In group A, Peripheral blood CD3 + , CD4 + , CD4 +/
·论 著·
6350.2016.10.017