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      标题:唾液淀粉酶活性影响因素教学实验改进探讨
      作者:李崇奇,蔡望伟
    (海南医学院生物化学与分子生物学教研室,海南 海口 571199)
      卷次: 2014年25卷11期
      【摘要】 唾液淀粉酶活性影响因素的探讨是现行大中专院校生物化学实验中的常见项目。然而由于不同
人群中唾液淀粉酶的活性相差较大,从而导致酶量很难控制。此外现行实验教材中该实验设计粗放,导致实验
结果与现行生物化学理论不一致。因而本文从酶含量的确定、温度、pH值、激活剂与抑制剂几个方面,对该实验
设计提出建议。

      【关键词】 唾液淀粉酶;酶活;实验教学

      【中图分类号】 R193.8 【文献标识码】 C 【文章编号】 1003—6350(2014)11—1688—02


Improvement of teaching experiment on factors influencing activity in salivary amylase.

LI Chong-qi, CAI
Wang-wei. Department of Biochemistry and Molecular Biology, Hainan Medical University, Haikou 571199, Hainan,
CHINA.
【Abstract】 Teaching experiment on salivary amylase activity is a common project of biochemical experiment
in current colleges. The amount of enzyme is difficult to be controlled because of the difference of salivary amylase ac-
tivity in different populations. In addition, the current experimental program is too simple, which leads to inconsisten-
cy between result and biochemistry theory. So this paper provides suggestions on the amount of enzyme, temperature,
pH, activator and inhibitor, to improve this experiment.

      【Key words】 Salivary amylase; Enzyme activity; Experiment teaching
基金项目:海南省教育厅教育科研项目(编号:Hjsk2012-56);海南医学院教育科研项目(编号:HYZ201305; HYZX201103)

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