标题：来氟米特对实验性变态反应性脑脊髓炎小鼠T细胞亚群及Th1型细胞因子的影响

      作者：袁正洲 1，李作孝 1，马勋泰 1，罗 斌 2，胡高云 2，杨 波 2，陈 琪 3
    (泸州医学院附属医院神经内科 1、介入科 2、肝胆外科 3，四川 泸州 646000)

      卷次： 2014年25卷4期

      【摘要】 目的 探讨来氟米特(Leflunomide，LEF)对实验性变态反应性脑脊髓炎(EAE)小鼠模型发病防治作
用及可能的免疫学机制。方法 将30只雌性C57BL/6小鼠随机分为EAE对照组及高、低剂量LEF防治组，每组
10只。通过注射髓鞘少突胶质细胞糖蛋白35-55 (MOG35-55)免疫介导构建EAE模型。从造模前3 d开始起EAE
对照组及高、低剂量LEF防治组分别予以生理盐水8 mg/(kg·d)、LEF 2 mg/(kg·d)灌胃，连续10 d。观察小鼠发病情
况及外周血中T细胞亚群及 IFN-γ、IL-2水平的变化。结果 LEF各防治组小鼠临床症状较轻，发病高峰期外周
血T细胞亚群CD4+、CD8+分布比例较EAE对照组明显升高(P<0.01)，CD4+/CD8+值明显降低(P<0.01)；发病高峰期外
周血 IL-2、INF-γ含量较EAE对照组明显降低(P<0.01)，且高剂量降低更明显(P<0.01)。结论 LEF对EAE具有防
治作用，效果与LEF剂量有关，其防治作用可能与调节T淋巴细胞亚群分布及抑制 IFN-γ、IL-2水平有关。

      【关键词】 实验性变态反应性脑脊髓炎；多发性硬化；来氟米特；T细胞亚群；Th1型细胞因子

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350(2014)04—0480—04


Effect of leflunomide on T cell subsets and Th1-type cytokines in experimental allergic encephalomyelitis mice.
YUAN Zheng-zhou 1, LI Zuo-xiao 1, MA Xun-tai 1, LUO Bing 2, HU Gao-yun 2, YANG Bo 2, CHEN Qi 3.
Department of
Neurology 1, Department of Interventional Therapy 2, Department of Hepatobiliary Surgery 3, the Affiliated Hospital of
Luzhou Medical College, Luzhou 646000, Sichuan, CHINA

【Abstract】 Objective To investigate the effect of leflunomide (LEF) on experimental allergic encephalomy-
elitis (EAE) mice and the possible immunological mechanisms. Methods Thirty female C57BL/6 mice were random-
ly divided into EAE control group, high and low dose leflunomide treatment group, with n=10 in each group. EAE
model was built by injection of myelin oligodendrocyte glycoprotein 35-55 (MOG35-55). From three days before
modeling, the control group, the high dose treatment group, and low dose treatment group were feed saline, lefluno-
mide 8 mg/(kg·d), leflunomide 2 mg/(kg·d) for 10 days, respectively. Incidence was observed in mice and peripheral
blood T cell subsets and IFN-γ, IL-2 levels were detected. Results Each treatment group have mild clinical symp-
toms, and peripheral blood T cell subsets CD4 + , CD8 + distribution rates were significantly higher than EAE control
group (P<0.01), while CD4+/CD8+ value was lower (P<0.01). The peripheral blood IL-2, INF-γ levels in high and low
dose treatment group were significantly lower than EAE control group (P<0.01), and the reduction in high dose treat-
ment group was more significant (P<0.01). Conclusion Leflunomide has preventive effect on EAE, and the effect is
dose related with the leflunomide. Its preventive effect may be related to the regulation of T lymphocyte subsets and
inhibition of IFN-γ, IL-2.

      【Key words】 Experimental allergic encephalomyelitis; Multiple sclerosis; Leflunomide; T cell subsets; Th1
type cytokines
·论 著·