标题：miR-146a在乳腺浸润性导管癌中的表达及临床意义

      作者：田南南，杨建新
    (广东药学院，广东 广州 510080)

      卷次： 2013年24卷24期

      【摘要】 目的 观察microRNA-146a (miR-146a)在乳腺浸润性导管癌及癌旁正常对照组织中的表达，探讨
miR-146a的表达与乳腺癌临床病理特征的关系及其对乳腺癌发生发展的影响。方法 采用组织芯片平台，应用
原位杂交方法检测 65例乳腺浸润导管癌及癌旁正常对照组织中miR-146a的表达。结果 乳腺浸润性导管癌
miR-146a阳性率低于癌旁正常对照组织(44.6% vs 66.2%，P<0.05)；乳腺浸润性导管癌中miR-146a的表达与淋巴
结转移有关联(P<0.01)，与患者年龄、肿瘤大小、组织学分级、TNM分期、ER、PR、c-erbB-2、ki67、p53、E-cadherin
过表达均无相关性(P>0.05)。结论 miR-146a在乳腺浸润性导管癌中表达下调，提示其可能作为重要的抑癌因
子参与乳腺癌的发生发展过程，并可能成为乳腺癌新的肿瘤标记物或预后因子。

      【关键词】 乳腺浸润性导管癌；miR-146a；临床病理特征；原位杂交

      【中图分类号】 R737.9 【文献标识码】 A 【文章编号】 1003—6350（2013）24—3599—03


Expression of miR-146a in breast infiltrative ductal carcinoma and its clinical significance.
TIAN Nan-nan,
YANG Jian-xin. Guangdong Pharmaceutical University, Guongzhou 510080, Guangdong CHINA

【Abstract】 Objective To investigate the expression of microRNA-146a (miR-146a) in breast infiltrative
ductal carcinoma and its clinicopathologic significance. Methods miR-146a expression was detected in 65 cases of
breast infiltrative ductal carcinoma and corresponding paracarcinoma nomal tissues on tissue microarray sections by in
situ hybridization. Results The expression rate of miR-146a in breast infiltrative ductal carcinoma was significantly
lower than that of the paracarcinoma nomal tissues (44.6% vs 66.2%, P<0.05). There were positive correlations be-
tween miR-146a expression and lymph node metastasis (P<0.01). There were no correlations between the expression
of miR-146a and age, size, histological grading, TNM stage, ER and PR status, c-erbB-2 over expression, ki-67 posi-
tive expression, P53 positive expression, E-cadherin positive expression. Conclusion The down-expression of
miR-146a in the breast infiltrative ductal carcinoma suggests that it may be involved in tumourgenesis of breast carci-
noma. miR-146a may become a novel tumormarker or prognostic factor for breast carcinoma．

      【Key words】 Breast infiltrative ductal carcinoma; microRNA-146a (miR-146a); Clinicopathologic characteris-