标题：MicroRNA-34a对尿路上皮癌细胞株5637

      作者：细胞迁移、侵袭和增殖的影响
    张 颖 1，张 超 2
(1.深圳市龙华新区人民医院手术室，广东 深圳 518100；
2.天津市肿瘤医院泌尿肿瘤科，天津 300060)

      卷次： 2013年24卷22期

      【摘要】 目的 研究microRNA-34a (miR-34a)对膀胱肿瘤细胞株5637细胞迁移、侵袭和增殖的影响。方法 在
5637细胞株中过表达miR-34a，运用小室迁移实验和小室侵袭实验检测细胞迁移力和侵袭力；运用血球计数板法和新
型四唑氮盐(MTS)法检测细胞增殖力。结果 过表达miR-34a后，5637细胞迁移力和侵袭力均下降(P<0.01)，细胞增
殖受抑(P<0.01)。结论 过表达miR-34能抑制膀胱肿瘤细胞株5637的迁移、侵袭和增殖。

      【关键词】 尿路上皮癌；迁移；侵袭；增殖；microRNA-34a

      【中图分类号】 R737.15 【文献标识码】 A 【文章编号】 1003—6350(2013)22—3277—04


Effects of MicroRNA-34a on the cell migration, invasion and proliferation of bladder tumor cell line 5637.
ZHANG Ying 1, ZHANG Chao 2.
1. Operating Room, People's Hospital of Longhua New District of Shenzhen, Shenzhen
518100, Guangdong, CHINA; 2. Department of Urology & Oncology, Tianjin Cancer Hospital, Tianjin 300060, CHINA

【Abstract】 Objective To evaluate the effects of microRNA-34a (miRNA-34a) on cell migration, invasion
and proliferation of bladder cancer cell line 5637. Methods miR-34a was overexpressed in bladder cancer cell line
5637. Transwell migration assay and transwell invasion assay were performed to predict cell migration ability and in-
vasion ability, respectively. Cell proliferation was assessed by cell counting assay and MTS assay. Results The over-
expression of MiR-34a significantly down-regulated cell migration and cell invasion of bladder cancer cell line 5637
(P<0.01), and significantly decreased cell proliferation (P<0.01). Conclusion The overexpression of microRNA-34a
inhibits cell migration, invasion and proliferation in bladder cancer cell line 5637.

      【Key words】 Urothelial carcinoma; Migration; Invasion; Proliferation; microRNA-34a
基金项目：天津医科大学附属肿瘤医院引进人才与博士启动基金(编号：B1208)