标题：慢性乙肝患者外周血CD4+CD25+Foxp3+调节性T细胞的检测及临床意义

      作者：邬艳波 1，蔡汉新 2，刘育琼 1，邓 欣 3
    (1.深圳市观澜人民医院感染科，广东 深圳 518110；
2.深圳市龙华新区卫生监督所医疗机构监督科，广东 深圳 518110；
3.深圳市第三人民医院肝病科，广东 深圳 518112)

      卷次： 2013年24卷19期

      【摘要】 目的 探讨自身抗体阳性慢性乙肝(CHB)患者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)的特
点。方法 自身抗体阳性的CHB患者20例、自身抗体阴性的CHB 20例、自身免疫性肝炎15例。用斑点免疫层
析法检测其抗单链DNA抗体(ss-DNA)、抗双链DNA抗体(ds-DNA)、抗核抗体(ANA)、可提取核抗原抗体谱
(ENA)、抗线粒体 IgM抗体(AMA)。流式细胞术检测外周血中的CD4+CD25+ Foxp3+调节性T细胞比例，并与20例
正常人对照组的上述指标进行比较。结果 自身抗体阳性CHB组CD4+CD25+T细胞较正常人无减低(P>0.05)，但
CD4+CD25+Foxp3+T细胞显著降低(P<0.05)；与自身抗体阳性CHB比较，自身抗体阴性CHB组CD4+CD25+T细胞和
CD4+CD25+Foxp3+T细胞均显著升高(P<0.05)，而自身免疫性肝炎情况则相反。结论 自身抗体阳性CHB患者
Treg细胞功能较正常人显著降低，而且比自身抗体阴性CHB患者的Treg细胞数量及功能均偏低。这可能是自
身抗体阳性CHB病情较重、反复发作的原因之一。

      【关键词】 自身抗体；慢性乙型肝炎；CD4+CD25+Foxp3+调节性T细胞

      【中图分类号】 R512.6+2 【文献标识码】 A 【文章编号】 1003—6350（2013）19—2872—03


Detection and clinical significance of CD4+CD25+Foxp3+ regulatory T cells in patients with autoantibody-positive
chronic hepatitis B.
WU Yan-bo 1, CAI Han-xin 2, LIU Yu-qiong 1, Den Xing 3. 1. Department of Infection, Shenzhen
Guanlan People's Hospital, Shenzhen 518110, Guangdong, CHINA; 2. Department of Medical Institution Supervision,
Shenzhen Guanlan Health Supervision Institute, Shenzhen 518110, Guangdong, CHINA; 3. Department of Hepatology,
Shenzhen Third People's Hospital, Shenzhen 518112, Guangdong, CHINA

【Abstract】 Objective To investigate the features of CD4+CD25+Foxp3+ regulatory T cell in patients with auto-
antibody-positive chronic hepatitis B. Methods Twenty patients with autoantibody-positive chronic hepatitis B
(CHB), 20 patients with autoantibody-negative CHB, and 15 patients with autoimmune hepatitis (AIH) were selected
as research subjects, and 20 healthy people were taken as the control group. Anti-single-stranded DNA antibody
(ss-DNA), anti-double-stranded DNA antibodies (ds-DNA), antinuclear antibody (ANA), extractable nuclear antigens
antibody (ENA), anti-mitochondrial IgM antibodies (AMA) were detected by dot immuno-chromatography. CD4+CD25+
Foxp3 + regulatory T cells were measured by using flow cytometry. Results Compared with healthy people, CD4 +
·论 著·doi:10.3969/j.issn.1003-6350.2013.19.1197