标题：支气管哮喘小鼠外周血CD8+CD28-T细胞群和CD8+CD56+T细胞群作用机制研究

      作者：黄琳惠，黄奕江，蔡兴俊，莫儒冰
    (海南省人民医院呼吸内科，海南 海口 570311)

      卷次： 2013年24卷18期

      【摘要】 目的 研究哮喘小鼠外周血CD8+CD28-T细胞与CD8+CD56+T细胞间的关系及两种细胞群在哮喘中的
作用。方法 30只BALB/c小鼠随机分为对照组和哮喘组，每组各15只。测定小鼠的气道反应性，对支气管肺
泡灌洗液行细胞学分类，观察肺组织的病理变化，流式检测小鼠外周血CD8+CD28-T细胞及CD8+CD56+T细胞的比
例，分析哮喘中两种细胞之间的相关性。结果 成功建立小鼠哮喘模型。①哮喘组小鼠BALF中细胞总数和嗜
酸性粒细胞(%)较对照组明显增高；②哮喘组小鼠外周血CD8+CD28-T细胞及CD8+CD56+T细胞比例均较对照组升
高；③ CD8+CD28-T细胞与CD8+CD56+T细胞数量上呈正相关(r=0.737，P=0.002)。结论 哮喘时外周血CD8+CD56+细
胞和CD8+CD28-细胞数目异常，两种细胞正相关，可能在哮喘发病中起重要作用。

      【关键词】 哮喘小鼠模型；气道反应性；支气管肺泡灌洗液；外周血；CD8+CD28-T细胞；CD8+CD56+T细胞

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2013）18—2658—03


Function and mechanism of CD8 +CD28- T cells and CD8 +CD28- T cells in peripheral blood of asthmatic mouse
model.
HUANG Lin-hui, HUANG Yi-jiang, CAI Xing-jun, MO Ru-bing. Department of Respiratory Medicine, People's
Hospital of Hainan Province, Haikou 570311, Hainan, CHINA

【Abstract】 Objective To examine the relationship between CD8 +CD28- and CD8 +CD56 + T cells in peripheral
blood of mice and the function of the two subsets in the development of asthma. Methods Thirty Balb/c mice were
randomly divided into two groups: asthma group, control group, with 15 mice in each group. The airway responsive-
ness were measured, bronchoalveolar lavage cytology was performed and lung tissue sections were prepared for histo-
pathologic examination to evaluate the airway inflammation, examine the fraction of CD8+CD28- and CD8+CD56+ T cells
by flow cytometry and correlation analysis. Results The mouse models established successfully. ① The total cell
number and the percentages of eosinophils in BALF of the asthmatic group were significantly higher than that in the
control group; ② The percentages of CD8 +CD28- and CD8 +CD56 + T cells in peripheral blood [(13.65±3.075) vs (4.11±
1.47)] in asthmatic group were significantly higher than that in control group [(10.32±2.318) vs (2.59±1.19)].③ There
was a strong positive correlation between CD8+CD28- and CD8+CD56+ T cells (r=0.737, P=0.002). Conclusion The frac-
tions of CD8+CD28- and CD8+CD56+ T cells are associated with asthma ongoing closely, and there is a strong positive cor-
relation between these two subsets.

      【Key words】 Airway responsiveness; Bronchoalveolar lavage fluid; Peripheral blood; CD8 +CD28- T cell; CD8 +
CD56+ T cell