标题：乙型肝炎患者前S1抗原与HBV-DNA和HBeAg的相关性研究

      作者：熊格，乔斌，李艳
    (武汉大学人民医院检验科，湖北 武汉 430060)

      卷次： 2016年27卷8期

      【摘要】 目的 探讨乙型肝炎患者前S1抗原(Pre-S1-Ag)与HBV-DNA、HBeAg的相互关系，并评价Pre-Sl-Ag
在反映乙型肝炎病毒复制方面的临床应用价值。方法 收集2014年1月至2015年1月经我院确诊为乙型肝炎患
者的血清标本248例，采用电化学发光法检测血清学标志物(HBsAg、抗-HBs、HBeAg、抗-HBe和抗-HBc)、酶联免
疫吸附试验(ELISA)检测Pre-S1-Ag、实时荧光定量聚合酶链反应(RT- PCR)检测HBV-DNA载量。同时对 50例健
康对照者也进行相同的检测。结果 248例乙型肝炎患者HBV-DNA、Pre-S1-Ag、HBeAg的阳性率分别为 62.5%
(155/248)、56.0% (139/248)、36.3% (90/248)；Pre-S1-Ag与 HBV-DNA存在相关性 (P<0.01，r=0.586)，Pre-S1-Ag与
HBeAg也存在相关性(P<0.01，r=0.459)，且 Pre-S1-Ag与HBV-DNA的相关性更密切；以乙肝病毒HBV-DNA≥
103copies/ml为判断乙肝病毒存在复制的标准，Pre-S1-Ag的灵敏度、特异度、阳性预测值、阴性预测值、阳性似然
比、阴性似然比和Youden指数分别为：83.9%、90.3%、0.94、0.77、8.7、0.18、0.74。结论 Pre-S1-Ag与HBeAg相比，
Pre-S1-Ag和HBV-DNA有较高的阳性符合率，能更准确地反映HBV的复制情况。

      【关键词】 乙型肝炎；前S1抗原；HBV-DNA；相关性

      【中图分类号】 R512.6+2 【文献标识码】 A 【文章编号】 1003—6350（2016）08—1225—03


Correlation between pre-S1 antigen and HBV-DNA, HBeAg in hepatitis B patients.
XIONG Ge, QIAO Bin, LI Yan.
Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, CHINA

【Abstract】 Objective To study the correlation between pre-S1 antigen (Pre-S1-Ag) and HBV-DNA, HBeAg
in hepatitis B patients, and to evaluate the clinical value of Pre-Sl-Ag in reflecting the replication of hepatitis B virus.
Methods Serum samples were collected from 248 patients with hepatitis B infection in our hospital from Jan. 2014 to
Jan. 2015. The serological markers (HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc) were detected by the electroche-
miluminescence immunoassay method, and Pre-S1-Ag was detected by enzyme-linked immunosorbent assay (ELISA).
HBV-DNA copies were detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Mean-
while, 50 healthy control subjects were also detected. Results The positive rates of Pre-S1-Ag, HBV-DNA, HBeAg
were 62.5% (155/248), 56.0% (139/248) and 36.3% (90/248), respectively. Significant correlation was found between
Pre-S1-Ag and HBV-DNA (P<0.01, r=0.586), Pre-S1-Ag and HBeAg (P<0.01, r=0.459), as well as between
Pre-S1-Ag and HBV-DNA. Taking HBV-DNA≥103 copies/mL as the judgment of HBV replication, the sensitivity,
specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), negative
likelihood ratio (NLR) and Youden index of Pre-S1-Ag were 83.9%, 90.3%, 0.94, 0.77, 8.7, 0.18 and 0.74, respective-
ly. Conclusion Compared with Pre-S1-Ag and HBeAg, Pre-S1-Ag and HBV have a higher positive coincident rate,
and can more accurately reflect the replication of HBV.

      【Key words】 Hepatitis B; Pre-S1 antigen; HBV-DNA; Correlation
·论 著·
6350.2016.08.006