标题：膀胱癌患者尿脱落细胞CK20的表达及临床诊断意义
作者：韩惟青
（湖南省肿瘤医院腹部外科，湖南 长沙 410013）
卷次：
2013年24卷13期
【摘要】 目的 观察尿脱落细胞CK20在膀胱癌患者中的表达情况，探讨其临床价值及意义。方法 采用
RT-PCR检测方法检测35例膀胱癌患者尿脱落细胞CK20的表达情况，并与30例非肿瘤疾病患者CK20的表达情
况进行对比分析。结果 35例膀胱癌患者尿脱落细胞CK20 mRNA阳性率为94.29%（33/35），而非肿瘤疾病患
者阳性率仅为3.33%（1/30），两组CK20 mRNA阳性率比较差异有显著统计学意义（P<0.01）。RT-PCR检测法检
测尿脱落细胞CK20 mRNA的敏感性为 94.26%，特异性为 96.67%。不同膀胱癌病理分期、分级的尿脱落细胞
CK20 mRNA表达阳性率比较差异无统计学意义（P>0.05）。结论 尿脱落细胞CK20在膀胱癌尿液中表达的敏
感率及特异性较高，是目前较为理想的一种膀胱肿瘤标记物，RT-PCR检测法检测尿脱落细胞中CK20有助于膀
胱癌的诊断。
【关键词】 膀胱癌；尿脱落细胞；CK20；RT-PCR检测法
【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350（2013）13—1951—03
Expression of CK20 in exfoliated urothelial cells of patients with bladder cancer and its significance in clinical
diagnosis.
HAN Wei-qing. Department of Abdominal Surgery, Hunan Provincial Tumor Hospital, Changsha 410013,
Hunan, CHINA
【Abstract】 Objective To observe the expression of CK20 in exfoliated urothelial cells of patients
with bladder cancer, and to investigate its clinical value and significance. Methods RT-PCR method was used
to detect the expression of CK20 in exfoliated urothelial cells of 35 patients with bladder cancer (the study
group), which were then compared with the expression level in 30 patients without neoplastic diseases (the con-
trol group). Results The positive rate of CK20 mRNA was 95.29% (33/35) in the study group and 3.33% (1/30)
in the control group, with statistically significant difference between the two groups (P<0.01). RT-PCR
showed that the CK20 mRNA sensitivity in exfoliated urothelial cells was 94.26% , and the specificity was
96.67% . The positive rate of CK20 mRNA in urine exfoliated cells had no statistically significant difference
among different pathological stages and grades of bladder cancer (P>0.05). Conclusion CK20 expression in
exfoliated urothelial cells of patients with bladder cancer has higher sensitivity and specificity, which is an ide-
al bladder tumor marker. The detection of CK20 in urine exfoliated cells by RT-PCR assay contributes to blad-
der cancer diagnosis.
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