标题：曼氏迭宫绦虫线粒体载体蛋白结构和功能的生物信息学分析

      作者：芦亚君，史大中，钟赛凤，甘秀凤，吕 刚
    (海南医学院热带医学与检验医学院病原生物学教研室，海南 海口 571199)

      卷次： 2013年24卷13期

      【摘要】 目的 应用生物信息学技术预测曼氏迭宫绦虫线粒体载体蛋白超家族的结构和功能，为进一步研
究曼氏迭宫绦虫的蛋白质提供理论依据。方法 将测得的曼氏迭宫绦虫成虫EST序列用ORF finder获取开放读
码框，Blast进行分析其结构域。应用分析工具 Protparam、InterProScan、protscale、SignalP-3.0、PSORT II、
BepiPred、TMHMM、VectorNTI Suite 9软件包、Phyre2分别进行该蛋白质的基本性质、结构域、疏水性、信号肽、亚
细胞定位、抗原表位、跨膜区及空间结构的预测及分析。结果 Blast预测该蛋白质为线粒体载体蛋白超家族，保
守功能域为线粒体二羧酸载体，含 294个氨基酸残基，理论分子量为 32 132.2 Da。与曼氏血吸虫进化地位最接
近；有1个信号肽位点和6个潜在的抗原表位。结论 曼氏迭宫绦虫线粒体二羧酸载体能够介导苹果酸、琥珀酸
等二羧酸的转运，使其跨越线粒体膜参与三羧酸循环，为虫体自身提供能量，可能是潜在的疫苗候选分子及药物
作用靶点。

      【关键词】 曼氏迭宫绦虫；线粒体载体蛋白；二羧酸载体；生物信息学分析

      【中图分类号】 R532.3 【文献标识码】 A 【文章编号】 1003—6350（2013）13—1879—04


Bioinformatics analysis for structure and function of mitochondrial carrier protein superfamily from Spirome-
tra mansoni.
LU Ya-jun, SHI Da-zhong, ZHONG Sai-feng, GAN Xiu-feng, LU Gang. Department of Pathogen Biology,
School of Tropical medicine and laboratory medicine, Hainan Medical University, Haikou 571199, Hainan, CHINA

【Abstract】 Objective To study the structure and function of mitochondrial carrier protein superfamily from
Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study. Methods Open
reading frame (ORF) of EST sequence from Spirometra mansoni was obtained by ORF finder and was translated into
amino acid by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools: Prot-
param, InterProScan, protscale, SignalP-3.0, PSORT II, BepiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2,
the structure and function of the protein were predicted and analyzed. Results The mitochondrial carrier protein su-
perfamily may be Spirometra mansoni mitochondrial dicarboxylate carrier (Sm DIC). The sequence contained 294 ami-
no acid residues and its theoretical molecular weight was 32003.4 Da. It had three full conserved functional domains
that was mito-carr superfamily, with the closest to evolutionary position of Schistosoma mansoni. It had a signal pep-
tide site and six potential antigen epitopes. Conclusion Sm DIC can mediate malic acid, succinic acid and other