标题：358例自然流产夫妇细胞遗传学分析

      作者：李远眺，李 勇
    (广西医科大学第四附属医院检验科遗传室，广西 柳州 545005)

      卷次： 2013年24卷12期

      【摘要】 目的 探讨自然流产与染色体异常之间的关系及其临床意义。方法 对358例有自然流产史的夫
妇进行外周血淋巴细胞培养，G显带，显微镜下计数20个分裂相，综合分析3~5个核型。染色体数目及结构异常
者，计数100个分裂相，加倍分析染色体核型。结果 358例自然流产夫妇中共检出染色体异常32例，异常率为
8.94%；其中男性15例，女性17例。常染色体异常22例，占6.14%；性染色体异常10例，占2.79%。结论 染色体
异常是导致自然流产的重要因素之一，对有自然流产史的夫妇进行染色体检查有助于病因诊断，提供临床指导，
达到优生优育的目的。

      【关键词】 自然流产；染色体异常；核型分析

      【中图分类号】 R446.11+3 【文献标识码】 B 【文章编号】 1003—6350（2013）12—1775—03


Cytogenetic analysis of 358 couples with spontaneous abortion.
LI Yuan-tiao, LI Yong. Genetics Room, Department
of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545005, Guangxi, CHINA

【Abstract】 Objective To explore the relationship between spontaneous abortion and chromosome anomaly
and its clinical significance. Methods A total of 358 couples who had a history of spontaneous abortion were en-
rolled and the lymphocytes were cultured. Karyotypes were analyzed by G-banding in all cases, 20 split-phases of
cells were counted under microscope and 3~5 karyotypes were comprehensively analyzed. For the couples with
abnormal chromosome number and structure, 100 split phases were counted and the karyotype was double ana-
lyzed. Results Of the 358 couples, 32 cases of chromosomal abnormalities (17 females and 15 males) were found,
with the overall incidence of 8.94%. Twenty-two cases were found with autosomic abnormality, accounting for 6.14%,
and 10 cases were found with sex chromosome abnormalities, accounting for 2.79%. Conclusion Chromosomal ab-
normality is an important cause in recurrent spontaneous abortion. For the couples with recurrent spontaneous abor-
tion, examination of the karyotype would help to identify the cause and provide medical advices, ultimately achieve
the purpose of prenatal and postnatal care.