标题：血清钙检测受负干扰的试剂污染因素探讨

      作者：雷震山 1，王 毅 2，吴少琴 1，钟 岸 2
    （1.深圳市保健委员会办公室，广东 深圳 518020；
2.深圳市孙逸仙心血管医院检验科，广东 深圳 518000）

      卷次： 2013年24卷8期

      【摘要】 目的 排查生化分析仪上对血清钙(Ca)检测产生负干扰的试剂交叉污染项目，并分析原因。方法 在
HITACHI 7600全自动生化分析仪上，将18项常规生化项目与Ca配对组合检测，筛选出干扰明显的施污染项目，
再将施污染试剂直接加入血清中，将试剂-血清混合物置生化仪上分析Ca浓度，根据Ca浓度是否明显偏离确定
该试剂是否存在干扰。对各试剂成分进行调研，分析造成Ca负干扰的因素。结果 TBIL（钒酸盐法）、DBIL（钒
酸盐法）、ALT、AST、HBD和GLU对Ca存在明显试剂负干扰，原因可能是EDTA、EGTA、柠檬酸盐等试剂添加剂
致Ca失活。结论 Ca的负干扰因素多而抗干扰能力较低，日常工作中应注意重点防范。

      【关键词】 钙；负干扰；交叉污染；螯合剂

      【中图分类号】 R446.11+2 【文献标识码】 A 【文章编号】 1003—6350（2013）08—1170—02


Reagent contamination factors of serum calcium detection with negative interference.
LEI Zhen-shan 1, WANG Yi 2,
WU Shao-qin 1, ZHONG An 2. 1. Shenzhen Veteran Cadres Health Office, Shenzhen 518000, Guangdong, CHINA; 2.
Department of Clinical Laboratory, Shenzhen Sun Yat-sen Cardlovascular Hospital, Shenzhen 518000, Guangdong, CHINA

【Abstract】 Objective To investigate reagent contamination factors of serum calcium (Ca) detection with
negative interference on biochemical analyzer, and to analyze the reasons. Methods On HITACHI 7600 automatic
biochemical analyzer, 18 routine biochemical projects and Ca paired combined detection were performed. Significant
interfere with the implementation of pollution projects were screened, and then the pollution reagents were directly
added to the serum. The reagent-serum mixture was analyzed for Ca concentration by biochemical analyzer. The appar-
ent deviation from the reagent interference was determined based on Ca concentration. The reagent contamination fac-
tors with negative interference were analyzed. Results TBIL (the vanadate Act), DBIL (vanadate law), ALT, AST,
HBD and GLU exerted obvious negative interference on Ca. The reason might be that to the application of EDTA, EG-
TA, citrate reagent induced inactivation of Ca. Conclusion Ca has lots of negative interference factors and low an-
ti-interference ability, which should be paid attention to in daily work.

      【Key words】 Calcium; Negative interference; Cross-contamination; Chelating agents