标题：常用梅毒抗体检测方法的比较

      作者：缪亚梅，王文鼎
    (姜堰市溱潼人民医院检验科，江苏 姜堰 225508）

      卷次： 2013年24卷8期

      【摘要】 目的 探讨不同梅毒抗体检测方学的敏感性和特异性。方法 利用梅毒螺旋体胶凝试验（TPPA）、
梅毒酶联免疫吸附试验（TP-ELISA）和梅毒螺旋体抗体快速血浆反应素试验（RPR）三种不同方法，对68份阳性
标本及80份阴性标本分别进行检测，比较其敏感性和特异性。结果 TPPA、TP-ELISA和RPR三法的灵敏度分
别为97.1%、94.1%、80.9%，特异性分别为97.5%、91.3%、88.8%，阳性预期值分别为97.1%、90.1%、85.9%，阴性预
期值分别为97.5%、94.8%、84.5%。与灵敏度和特异性均较好的TPPA比较，RPR敏感性和特异性较低，敏感性差
异有统计学意义(P＜0.01)，特异性间差异有统计学意义(P＜0.05)。结论 TPPA不适合大规模的血液筛查，适用
于对筛查后的阳性标本进行梅毒抗体的确证试验；TP-ELISA是目前筛查梅毒的理想方法；RPR检测梅毒非特异
性抗体，用于疗效观察及过筛试验。

      【关键词】 梅毒螺旋体抗体；血清学检验；TPPA；TP-ELISA；RPR

      【中图分类号】 R446.62 【文献标识码】 A 【文章编号】 1003—6350（2013）08—1168—03


Comparison of detection methods of syphilis antibodies.
MIAO Ya-mei, WANG Wen-ding. Department of Clinical
Laboratory, Qintong People's Hospital of Jiangyan City, Jiangyan 225508, Jiangsu, CHINA

【Abstract】 Objective To explore the sensitivity and specificity of different methods in detecting the anti-
body of treponema pallidum (TP). Methods The antibody of TP in 68 positive and 80 negative specimen were de-
tected using treponema pallidum particle agglutination assay (TPPA), TP-ELISA and rapid plasma reagin assay (RPR),
respectively. The sensitivity and specificity of the three methods were compared. Results The sensitivity of TPPA,
TP-ELISA, RPR were 97.1%, 94.1%, 80.9%, and the specificity was 97.5%, 91.3%, 88.8%, respectively. The positive
predictive value were 97.1%, 90.1%, 85.9%, and the negative predictive value were 97.5%, 94.8%, 84.5%, respective-
ly. Compared with TPPA with relative good sensitivity and specificity, RPR had significantly lower sensitivity and
specificity (P<0.01, P<0.05). Conclusion TPPA is suitable not for large-scale screening of blood, but confirmation
test of syphilis antibody in positive specimens. TP-ELISA is the ideal method for screening of syphilis. RPR is suit-
able for the detection of non-specific antibodies, observation of clinical efficacy and sieve test.

      【Key words】 Treponema pallidum antibody; Serological test; TPPA; TP-ELISA; RPR